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A molecular identification method of cotton stigma exposed gene

A technology for stigma exposure and molecular identification, which is applied in the field of stigma exposure gene identification and molecular marker assisted breeding, can solve the problems of large cotton fields, manpower and financial resources, low probability, and long life of stigma exposure materials, so as to speed up the selection process. Breeding and improvement process, easy to operate, fast results

Inactive Publication Date: 2019-09-10
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the traditional breeding program relying on phenotypic identification, self-identification is required after each backcross to ensure that the stigma-exposed gene is not lost in the process of transformation and improvement, while the stigma-exposed gene is affected by two pairs of recessive Genetic control, the single plant with exposed stigma has a low probability of appearing in the segregated population, which requires a lot of cotton fields, manpower and financial resources
It takes a long time to select and improve materials with exposed stigmas by conventional breeding techniques

Method used

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  • A molecular identification method of cotton stigma exposed gene
  • A molecular identification method of cotton stigma exposed gene

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Embodiment Construction

[0038] The present invention will be further described in detail below in conjunction with the embodiments.

[0039] Those skilled in the art will understand that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention. If no specific technology or condition is indicated in the examples, the technology or condition described in the literature in the field or the product specification is used. The reagents or instruments used without the manufacturer's indication are conventional products that can be obtained through purchase.

[0040] Tris-Cl: Tris(hydroxymethyl)aminomethane-HCl; EDTA: Ethylenediaminetetraacetic acid; SDS: sodium dodecyl sulfate; PVP: polyethylene Polyvinylpyrrolidone

[0041] All reagents were purchased from: Sangon Bioengineering (Shanghai) Co., Ltd.; purity: analytical grade.

[0042] 1. DNA extraction

[0043] Step 1, take the sea island cotton variety Xinhai 18 w...

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Abstract

The invention belongs to the technical field of biology, and relates to a molecular identification method of CGSS (cotton germplasm with stigma showing), particularly gene-specific primer markers for identifying a CGSS-gene-containing cotton strain, wherein the nucleotide sequences are disclosed as SEQ ID NO.1, 2, 3 and 4. The gene-specific markers are utilized to identify the individual plants carrying CGSS gene in the population. The method has the advantages of high identification speed and high accuracy, is simple to operate, and is very suitable for early indoor identification and molecular-marker-assisted selective breeding of CGSS materials.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular identification method for stigma exposed gene identification and molecular marker-assisted breeding. Background technique [0002] Exposed stigma refers to a mutant trait of floral organs in cotton. It is manifested as soon after the plant buds (usually 12 to 20 days), the stigma stretches out and is higher than the corolla, while the stamens are still wrapped in the corolla, and the loose powder is not exposed until the flowering stamens, and the upper half (stigma base) stamen anthers are often The performance is necrotic, resulting in difficult stigma pollination and low boll formation rate. Due to the exposed stigma, this germplasm is also called dioecious. Such mutants often appear in the offspring of sea-land hybrids of cotton. Previous studies have shown that the stigma exposure trait is controlled by two pairs of recessive genes, that is, the stigma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895
Inventor 张永山陈伟姚金波李燕褚丽
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI