Preparation of hydrogen peroxide detection colorimetry/fluorescent paper chip in cancer cell

A hydrogen peroxide detection, paper chip technology, applied in the direction of color/spectral characteristic measurement, fluorescence/phosphorescence, material analysis by observing the influence of chemical indicators, etc., can solve the problem of low sensitivity, avoid long-term contact, Fast and visual inspection of the effect

Inactive Publication Date: 2017-01-25
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, colorimetric biosensors can only perform intuitive and visual determination of the analyte, and because of their low sensitivity, they can only perform semi-quantitative or qualitative detection

Method used

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  • Preparation of hydrogen peroxide detection colorimetry/fluorescent paper chip in cancer cell
  • Preparation of hydrogen peroxide detection colorimetry/fluorescent paper chip in cancer cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of colorimetric / fluorescent paper chip for detection of hydrogen peroxide in MCF-7 cells:

[0027] (1) Design on the computer as attached figure 1 The hydrophobic wax-printed area and the hydrophilic working area of ​​the colorimetric / fluorescent paper chip shown, the diameter of the hydrophilic area 1 and the application area of ​​the paper chip on the right is 6 mm, the diameter of the hydrophilic area 2 is 4 mm, and the diameter of the left The gray area of ​​the paper chip is the hole that completely corresponds to the hydrophilic area of ​​the paper chip on the right. The two holes are connected by a hydrophilic channel with a length of 4 mm and a width of 2 mm;

[0028] (2) Print the hydrophobic pattern on the paper chip designed in step (1) with a wax printer, and then heat the obtained paper chip at 120-150 ºC for 1-2 min to melt the wax and form a hydrophobic layer;

[0029] (3) Using a laser cutting machine, cut the paper chip obtained in step (2)...

Embodiment 2

[0035] The preparation steps are the same as in Example 1, except that the cancer cells used are A549 cells, and the fluorescent substance used is carbon dots.

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Abstract

The invention discloses a preparation method of a colorimetry/fluorescent paper chip for detecting hydrogen peroxide in a cancer cell. The method comprises the following steps: using a wax printing technology to prepare a hydrophobic area, a hydrophillic area and a hollow passageway on the paper chip, and cutting the hollow passageway through a laser cutting machine; growing cerium dioxide on a working area 2, wherein cerium dioxide with the enzyme-like property become saffron yellow in the presence of hydrogen peroxide, thus realizing visual colorimetric detection. Because hydrogen peroxide can replace DNA absorbed on cerium dioxide, high sensitive fluoremetry is realized through the 'off-on' of a fluorescence signal.

Description

technical field [0001] The invention relates to the technical field of low-cost, easy-to-carry and visualized analysis and detection of hydrogen peroxide in cancer cells, and more specifically to the preparation of a colorimetric / fluorescent paper chip that can be used for detection of hydrogen peroxide in cancer cells. Background technique [0002] Hydrogen peroxide is one of the most stable reactive oxygen species. It is an important intracellular signaling molecule, mainly regulating DNA damage, protein synthesis and cell apoptosis. At low concentrations, hydrogen peroxide, as a ubiquitous intracellular second messenger, can activate signal bridges to promote cell proliferation, differentiation and migration, and regulate and protect cells. However, when the concentration of hydrogen peroxide is overexpressed, it will cause the modification of DNA bases, such as the oxidation of purines and pyrimidines and the breakage of chains, etc., thereby causing cell mutation and ev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/25G01N21/78
CPCG01N21/25G01N21/64G01N21/6428G01N21/78
Inventor 梁琳琳葛慎光于京华兰飞飞李丽
Owner UNIV OF JINAN
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