Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Dunaliella phytoene dehydrogenase gene promoter and application thereof

A technology of Dunaliella phytoene and lycopene is applied in the construction of a cloning system, the field of Dunaliella phytoene dehydrogenase gene promoter, and achieves the effect of high electroporation efficiency

Inactive Publication Date: 2017-02-01
SOUTH CHINA UNIV OF TECH
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the problem that exogenous promoters are used for gene expression in the existing Dunaliella, the object of the present invention is to provide a Dunaliella phytoene dehydrogenase gene promoter

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Dunaliella phytoene dehydrogenase gene promoter and application thereof
  • Dunaliella phytoene dehydrogenase gene promoter and application thereof
  • Dunaliella phytoene dehydrogenase gene promoter and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Use gene-specific primers pPdSP1 (5'-CCTCTAGTTCAATCCAAGGTGTTCAT-3'), pPdSP2 (5'-ACAAAGAGCCACAGCCCACTAA-3') and pPdSP3 (5'-ATTGGTTCAGAAGAGCCGTGAGTCC-3'), and AP degenerate primers for genome walking. As a result, the target band was obtained with the AP2 degenerate primer, such as figure 1 show.

[0034] AP degenerate primers were purchased from Dalian Bao Biological Company.

[0035] 1) Take an appropriate amount of Dunaliella genome as a template (the amount of DNA in the optimal reaction of different species is not the same, please refer to the note *1 below for the actual amount), use AP (any one of the four types, the following uses AP1 as an example) As the upstream primer, pPdSP1 was used as the downstream primer, and the 1st PCR reaction was performed.

[0036] 1st PCR reaction system:

[0037] Template (genomic DNA) 1~2μL dNTP Mixture (2.5mM each)* 8μL 10×LA PCR Buffer II (Mg 2+ plus)*

5μL TaKaRa LA Taq (5U / μL)* 0.5μL ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a dunaliella phytoene dehydrogenase gene promoter and application thereof. The gene sequence of the dunaliella phytoene dehydrogenase gene promoter is represented by SEQ ID NO: 1. The phytoene dehydrogenase gene promoter obtained by cloning dunaliella can be applied to starting genes of microalgae such as the dunaliella, and is different from a common CaMV35s general promoter, so that transferring of a dunaliella endogenous promoter is really realized; the promoter can achieve the effect of transient expression of exogenous genes through verification. The promoter disclosed by the invention can be further applied to the research on a regulation factor of the phytoene dehydrogenase gene promoter. Meanwhile, compared with a method, such as Yu Sun, of electric transfer of dunaliella, an electric shock transfer method optimized by the experiment is higher in electric transfer efficiency. The amount of green fluorescent proteins in the optimized transfer effect in the visual field is larger than that of the green fluorescent proteins in the transfer effect which is not optimized, so that the transfer efficiency is greatly improved.

Description

technical field [0001] The invention relates to the field of molecular cloning, and relates to a construction cloning system of a plant expression vector, in particular to a Dunaliella phytoene dehydrogenase gene promoter and application thereof. Background technique [0002] Phytoene dehydrogenase (PDS) is a key enzyme in the plant carotenoid metabolic pathway, regulating the conversion of phytoene to ζ-carotene. At present, the cDNA sequences of PDS genes of some plants and microalgae have been cloned and studied one after another. In plants with carotenoids as the main pigment species in flowers or fruits such as tomato, citrus, gentian, calendula, etc., the expression of PDS gene can promote the accumulation of carotenoids in flowers and fruits. Contents of the invention [0003] In order to overcome the problem that exogenous promoters are used for gene expression in the existing Dunaliella, the object of the present invention is to provide a Dunaliella phytoene dehy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/113C12N15/79C12N15/67
CPCC12N9/001C12N15/67C12N15/79C12Y103/99029C12N2800/60
Inventor 姜建国陈浩宏王天星
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com