Method for detecting nanoparticles adsorbed on surface of biofilm

A nanoparticle and surface adsorption technology, applied in measuring devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of difficult adsorption process, high detection limit, low sensitivity, etc., and achieve high sensitivity, easy operation, and intuitive expression of results Effect

Inactive Publication Date: 2017-02-01
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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Problems solved by technology

[0003] At present, the commonly used methods for detecting the adsorption/absorption of nanoparticles mainly include UV spectrophotometry, flow cytometry,

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  • Method for detecting nanoparticles adsorbed on surface of biofilm
  • Method for detecting nanoparticles adsorbed on surface of biofilm
  • Method for detecting nanoparticles adsorbed on surface of biofilm

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[0017] The present invention will be further described below in conjunction with specific examples, but the present invention is not limited to the following examples.

[0018] A method for detecting adsorbed nanoparticles on the surface of a biofilm according to the present invention, the method adds fluorescent nanoparticles to the biofilm attached to the growth of the cell slide, that is, the nanoparticles are combined on the surface of the biofilm, and the light is fully absorbed by the biofilm. After reflection, the evanescent wave generated on the large numerical aperture objective lens excites the nanoparticles to generate fluorescence, observes the biofilm area where the nanoparticles are adsorbed, and captures the number of fluorescent bright spots in the target area through the detector photomultiplier charge-coupled element to characterize the integrity of the nanoparticles in the biological The adsorption and permeation process on the surface of the membrane, the sp...

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Abstract

The invention discloses a method for detecting nanoparticles adsorbed on the surface of a biofilm. According to the method, nanoparticles capable of sending out fluorescence are added in a biofilm attached and grown on a cell slide and thus bonded on the surface of the biofilm, evanescent waves produced on a large numerical aperture objective lens after total reflection of light rays excite the nanoparticles to produce fluorescence, the nanoparticle adsorbed biofilm region is observed, and the adsorptive percolation process is characterized by the number of fluorescent light spots in an EMCCD (electron-multiplying charge-coupled device) target acquisition region. The nanoparticles adsorbed on the surface of the biofilm can be detected through the method; and the method has the advantages of simple operation process, high detection sensitivity, direct-viewing result expression and the like.

Description

technical field [0001] The invention provides a method for detecting adsorbed nanoparticles on the surface of a biofilm. Background technique [0002] As a new type of pollutant, nanoparticles are widely used in paints, cosmetics and other products; under natural conditions, many materials will also be converted into nanoparticles due to natural conditions such as weathering, light, etc., because of its small size and easy Migration, floating in the environmental medium, large specific surface area, easy to absorb toxic substances and other characteristics, easy to enter plant and animal cells, and pass along the food chain, posing a huge threat to environmental safety and human health. Biofilm is an important carrier in the process of pollutant removal, and the study of the adsorption process of biofilm on nanoparticles is of great significance to the removal of nanoparticle pollutants in the environment. The development of ultra-sensitive, fast and intuitive detection met...

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 潘响亮赵佳玥张道勇宋文娟
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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