Device and method for determining toxicity of Bactrocera cucurbitae feeding pesticide

Abstract

The invention provides a method for determining the toxicity of Bactrocera cucurbitae feeding pesticide. The method comprises the following steps: introducing 20 to 30 heads of Bactrocera cucurbitae which are hungry for 24 h 3 to 5 days later after emergence into a plastic box through an insect suction pipe and a cloth barrel; diluting Abamectin and a Trichlorfon technical material by acetone into 100 mg / L respectively as mother solutions; preparing a water solution containing 5% honey and 0.5% Twain-80; diluting the medicament mother solutions into five series of working concentrations by using the prepared water solution, taking a culture dish, putting absorbent paper, and using a liquid-moving machine to dropwise add the prepared solutions with five series of working concentrations onto the absorbent paper; using tweezers to put the culture dish to in the plastic box through the cloth barrel; putting the plastic box in an incubator at a specific environment; after 24 hours, taking out the incubator, and feeding with normal feeding food; and counting the death rate. The invention also provides a device for determining the toxicity of the Bactrocera cucurbitae feeding pesticide. Compared with the prior art, the method and the device have the beneficial effects that the operation is simple and the device is easy to manufacture and can be recycled.

Description

technical field [0001] The invention relates to a device and method for measuring the toxicity of melon fruit fly feeding poison, which belongs to the research field of insect toxicity measurement technology. Background technique [0002] Bactrocera (Zeugodacus) cucurbitae, belonging to Tephritidae, is widely distributed in subtropical regions and is an important invasive pest. It lays eggs in Lucaceae and Solanaceae plants such as pumpkin, melon, bitter gourd, watermelon, peach and tomato, etc., and spreads with its transportation. In recent years, with the expansion of the planting area of ​​melons and fruits and vegetables and the extension of the planting cycle, the harm of Bacteralis melons is increasing. According to statistics, melon fruit fly, Bactrocera dorsalis and Bactrocera dorsalis only cause economic losses of up to 12.987 billion yuan to Guangdong Province every year. At present, the control of fruit fly pests in actual production is still dominated by chemi...

AI Technical Summary

Problems solved by technology

The determination of drug resistance virulence is the basis for understanding the resistance status of field pest populations and controlling drug resistance. At present, the methods of drug resistance virulence determination mainly include drug film method, drip method and liquid immersion method, etc., but these are not suitable for food. Toxic agent is to the determination of the virulence of fruit flies such as melon fly, therefore, it is very necessary to develop a kind of method for measuring the toxicity of melon fruit fly feeding poison for being engaged in the research of this aspect

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