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A kind of detection method of isoprocarb

A detection method and technology of isoprocarb, applied in the field of analysis and detection, can solve the problems of complicated operation and long analysis time, and achieve the effects of short analysis time, simple operation and accurate detection results.

Active Publication Date: 2018-05-11
WUXI X RES PROD DESIGN & RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the industry standards or enterprise standards use gas chromatography to determine the content of isoprocarb, which is relatively cumbersome to operate and takes a long time to analyze

Method used

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  • A kind of detection method of isoprocarb
  • A kind of detection method of isoprocarb
  • A kind of detection method of isoprocarb

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Experimental program
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Effect test

Embodiment 1

[0024] In the embodiment of the present invention, a kind of detection method of isoprocarb comprises the following steps:

[0025] 1) Weigh the sample to be tested in a centrifuge tube, add the extraction solution into the centrifuge tube, vortex, oscillate, ultrasonic (ultrasonic power is 400W, ultrasonic time is 10min), centrifuge (centrifugal speed is 2000r / min, centrifugation time is After 2min), take 5ml of the above centrifuged supernatant in a 15ml centrifuge tube, and add 0.5g of diatomaceous earth to the centrifuge tube at the same time, fully vortex, shake, and centrifuge (centrifugation speed is 2000r / min, centrifugation time After 2min), take 2ml of the centrifuged supernatant and purify it with an ion-exchange solid-phase extraction column; activate the ion-exchange solid-phase extraction column with 4ml of methanol and 4ml of water in turn, rinse with 5ml of phosphate buffer solution, and then sequentially Add 5ml of water and 0.5ml of methanol for rinsing, use ...

Embodiment 2

[0034] In the embodiment of the present invention, a kind of detection method of isoprocarb comprises the following steps:

[0035] 1) Weigh the sample to be tested in a centrifuge tube, add the extraction solution into the centrifuge tube, vortex, oscillate, ultrasonic (ultrasonic power is 1200W, ultrasonic time is 90min), centrifuge (centrifugal speed is 10000r / min, centrifugation time is After 20min), take 10ml of the above centrifuged supernatant in a 15ml centrifuge tube, and add 1.5g of diatomaceous earth to the centrifuge tube at the same time, vortex, shake, and centrifuge (centrifugation speed is 10000r / min, centrifugation time After 20min), take 5ml of the centrifuged supernatant and purify it with an ion-exchange solid-phase extraction column; activate the ion-exchange solid-phase extraction column with 6ml of methanol and 6ml of water in turn, rinse with 8ml of phosphate buffer solution, and then sequentially Add 8ml of water and 2ml of methanol for rinsing, use 8m...

Embodiment 3

[0044] In the embodiment of the present invention, a kind of detection method of isoprocarb comprises the following steps:

[0045] 1) Weigh the sample to be tested in a centrifuge tube, add the extraction solution into the centrifuge tube, vortex, oscillate, ultrasonic (ultrasonic power is 600W, ultrasonic time is 30min), centrifuge (centrifugal speed is 3000r / min, centrifugation time is After 5min), take 6ml of the above centrifuged supernatant in a 15ml centrifuge tube, and add 0.8g of diatomaceous earth to the centrifuge tube at the same time, fully vortex, shake, and centrifuge (centrifugation speed is 3000r / min, centrifugation time After 5min), take 3ml of the centrifuged supernatant and purify it with an ion-exchange solid-phase extraction column; activate the ion-exchange solid-phase extraction column with 5ml of methanol and 5ml of water in turn, rinse with 6ml of phosphate buffer solution, and then sequentially Add 6ml of water and 1ml of methanol to rinse, use 6ml o...

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Abstract

The invention discloses a detection method of mobucin. The method comprises the following steps: putting a sample to be detected and an extracting solution into a centrifuge tube, carrying out vortex treatment, oscillation, ultrasonic treatment and centrifugation, putting the centrifugated supernate into a centrifuge tube, adding diatomite into the centrifuge tube, carrying out vortex treatment, oscillation and centrifugation, taking the centrifugated supernate, and purifying the centrifugated supernate through an ion exchange solid-phase extraction column; sequentially activating the ion exchange solid-phase extraction column with methanol and water, rinsing with a phosphate buffer solution, water and methanol, eluting the substance to be detected in the ion exchange solid-phase extraction column by using a formic-acid-containing methanol solution, blow-drying with nitrogen, and dissolving the residues in a formic-acid-containing acetonitrile-water solution; and carrying out liquid chromatography quantitative detection with a high performance liquid chromatograph and an ultraviolet detector by using a peak-area external standard process. The method has the advantages of higher sensitivity, short analysis time and accurate detection result, implements quick and accurate determination on mobucin, and is simple to operate.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a detection method of isoprocarb. Background technique [0002] Isoprocarb, also known as Yechansan and Meipucarb, is a kind of insecticide with both contact and systemic effects. It belongs to a cholinesterase inhibitor. It is an insecticide with contact and stomach poisoning effects. , Short residual effect period, used to control rice planthoppers, rice leafhoppers, aphids, bedbugs, etc. in rice, cocoa, vegetables, sugar cane and other crops. It is safe to natural enemies of planthoppers and spiders, but harmful to bees. Most industry standards or enterprise standards use gas chromatography to determine the content of isoprocarb, which is relatively cumbersome to operate and takes a long time to analyze. Therefore, it is necessary to establish a detection method that can accurately and quickly detect isoprocarb simultaneously, improve the quality detection eff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88G01N2030/884
Inventor 蒋韦艳刘金杰吴敏芳徐静赵春城胡勇朱倩倩
Owner WUXI X RES PROD DESIGN & RES