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The new application of polysaccharides from chrysanthemi to promote the synthesis of atractylodes lactones in Atractylodes macrocephala

The technology of Atractylodes Rhizoma Lactone and Wild Chrysanthemum is applied in the field of agricultural biology, which can solve the problems of increasing quality, and achieve the effects of low cost, improving the authentic quality of Atractylodes Rhizoma, and improving the value of development and utilization.

Active Publication Date: 2020-10-09
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the relationship between it and secondary metabolites has not yet been reported. Studies have found that polysaccharides can promote the metabolic accumulation of Atractylodes macrolides in Atractylodes macrocephala, improve the medicinal components of Atractylodes macrocephala, and increase the quality

Method used

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  • The new application of polysaccharides from chrysanthemi to promote the synthesis of atractylodes lactones in Atractylodes macrocephala
  • The new application of polysaccharides from chrysanthemi to promote the synthesis of atractylodes lactones in Atractylodes macrocephala

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1: the extraction method of wild chrysanthemum polysaccharide

[0052] (1) Collect the flowers of C.boreale Mak. and dry them in an oven, crush them, pass through a 100-mesh sieve, and weigh 1 kg for later use;

[0053] (2) Add sherwood oil of 6-8 times the volume of the chrysanthemum powder to the above-mentioned wild chrysanthemum powder, reflux 1-2 times, each time for 1-2 hours, take out, filter, and heat the filter residue in a water bath to evaporate the remaining petroleum ether. Obtain the decolorized wild chrysanthemum powder;

[0054] (3) Add the pure water of 18-20 times the volume of the wild chrysanthemum powder to the decolorized wild chrysanthemum powder, decoct 2-3 times, each 2-3 hours, take the juice and remove the residue, combine the filtrate, filter, Obtain the aqueous extract solution of wild chrysanthemum;

[0055] (4) concentrating the chrysanthemum aqueous extract solution under reduced pressure and low temperature to a specific gra...

Embodiment 2

[0059] Embodiment 2: the preparation of aqueous solution

[0060] Take the chrysanthemum polysaccharide extracted in Example 1, add appropriate amount of water to prepare an aqueous solution containing 100 g / L of chrysanthemum polysaccharide, shake well, and seal it for storage. Spraying and spraying on the seedling stage of Atractylodes macrocephala, compared with the control group, can significantly increase the content of atractylodes lactone.

Embodiment 3

[0061] Embodiment 3: the application of powder

[0062] Chrysanthemum polysaccharide (purity: 98%) was prepared according to the method in Example 1, dried, processed into fine powder, and sealed for storage. Before use, it was formulated into an aqueous solution containing 50 g / L of polysaccharides from Chrysanthemum chrysanthemum and sprayed on the leaves of Atractylodes macrocephala. Compared with the control group, the content of atractylodes lactones could be significantly increased.

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Abstract

The present invention discloses new chrysanthemum indicum uses for promoting the atractylodes macrocephala koidz growth stage and increasing the atractylenolide content accumulation. According to the present invention, the atractylodes macrocephala koidz polysaccharide can be prepared into the plant inducer so as to promote the metabolism accumulation of the atractylenolide component in the atractylodes macrocephala koidz plant, improve the efficacy component of the atractylodes macrocephala koidz, and increase the quality of the atractylodes macrocephala koidz; and the inducer is added with the commonly-used pharmaceutical excipient in the auxiliary manner so as to prepare the dosage forms such as an aqueous agent, powder, and the like.

Description

technical field [0001] The invention relates to chrysanthemum polysaccharides for promoting the accumulation of atractylodes atractylodes lactone in the growth period and belonging to the field of agricultural biotechnology. Background technique [0002] Atractylodes macrocephala is the dry rhizome of the perennial herb Atractylodes Atractylodes in the family Asteraceae, mainly produced in Zhejiang, Anhui, Hunan, Jiangxi and other places. Among them, Atractylodes atractylodes is of good quality, and Atractylodes lactones are the main pharmacodynamics of Atractylodes atractylodes. It has obvious prevention and control effects on human gastric cancer and intestinal diseases. The low yield of secondary metabolites is one of the core issues restricting the large-scale application of natural products. Although domestic and foreign researchers have conducted research on the low yield of secondary metabolites, including the selection of superior strains, the transformation of cult...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/16A01P21/00C05G3/00
Inventor 田薇陈磊杜宁宁
Owner ZHEJIANG FORESTRY UNIVERSITY
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