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Batch high-density fermentation method for bread yeast by using self-priming reactor

A high-density fermentation and baker's yeast technology, applied in the field of biological fermentation engineering, can solve problems such as unreasonable use of foam and fermentation failure, and achieve the effects of saving fermentation costs, ensuring fermentation power, and simplifying fermentation-related operation steps

Active Publication Date: 2017-02-15
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in high-density large-scale fermentation, the generation of a large amount of foam and the unreasonable use of antifoaming agents will also lead to fermentation failure

Method used

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  • Batch high-density fermentation method for bread yeast by using self-priming reactor
  • Batch high-density fermentation method for bread yeast by using self-priming reactor
  • Batch high-density fermentation method for bread yeast by using self-priming reactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Cultivate with 1000mL shake flask with wort liquid medium (pH4.8), the amount of liquid in the shake flask is 25% to 30%, and the inoculum size is 10%. 12h, prepare 2.5L of seed solution;

[0035] (2) Inoculate the above-mentioned seed liquid into the bottom water containing 12.5L (vitamin B 1 0.2g, vitamin B 5 0.2g, vitamin B 6 0.2g, biotin 0.1g) in the 50L self-priming reactor, calibrate the dissolved oxygen electrode DO as 100% at a moment before inoculation;

[0036] (3) The amount of molasses added is 2.3 times the amount of molasses added according to the current dry weight of yeast in the self-priming reactor. 2 hours before the end of fermentation, reduce the amount of molasses added by 50% to 60%, until the end of fermentation, stop feeding, urea The feeding rate is 8g / h, stop feeding after 20h, the feeding rate of ammonium dihydrogen phosphate is 3g / h, stop feeding after 16h, add 25g MgSO every 8h 4 , added 3 times in total;

[0037] (4) Control the v...

Embodiment 2

[0043] (1) Cultivate with 1000mL shake flask with wort liquid medium (pH4.8), the amount of liquid in the shake flask is 25% to 30%, and the inoculum size is 10%. 12h, prepare 2.5L of seed solution;

[0044] (2) Inoculate the above-mentioned seed liquid into the bottom water containing 12.5L (vitamin B 1 0.2g, vitamin B 5 0.2g, vitamin B 6 0.2g, biotin 0.1g) in the 50L self-priming reactor, calibrate the dissolved oxygen electrode DO as 100% at a moment before inoculation;

[0045] (3) The amount of molasses added is 2.3 times the amount of molasses added according to the current dry weight of yeast in the self-priming reactor. 2 hours before the end of fermentation, reduce the amount of molasses added by 50% to 60%, until the end of fermentation, stop feeding, urea The feeding rate is 8g / h, stop feeding after 20h, the feeding rate of ammonium dihydrogen phosphate is 3g / h, stop feeding after 16h, add 25g MgSO every 8h 4 , added 3 times in total;

[0046] (4) Control the...

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PUM

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Abstract

The invention discloses a batch high-density fermentation method for bread yeast by using a self-priming reactor. The method is simple and convenient in operation; the fermentation efficiency is effectively improved while the vitality of mycelium can be maintained. According to the batch high-density fermentation method for the bread yeast by using the self-priming reactor, disclosed by the invention, the self-priming reactor is utilized, molasses, urea and ammonium dihydrogen phosphate are used as nutrient sources for weighing, and flow feeding is performed for high-density fermentation on the bread yeast.

Description

technical field [0001] The invention relates to a method for high-density cultivation of baker's yeast, more specifically to a batch-type high-density fermentation method for baker's yeast using a self-priming reactor, and belongs to the technical field of biological fermentation engineering. Background technique [0002] Yeast is a single-celled microorganism with high amino acid content, about 50% protein, rich in B vitamins, rich in enzymes and a variety of physiologically active substances with high economic value. Yeast is a microorganism with great application value. In the food industry, it is widely used as an excellent leavening agent and nutrient for human staple food such as bread, steamed buns, biscuits and cakes; in the pharmaceutical industry, yeast is used as an auxiliary therapeutic agent Or used as a culture medium; in the field of basic research, yeast is the best model material for microbiologists and geneticists to carry out basic research; in addition, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/18C12R1/865
CPCC12N1/18
Inventor 洪厚胜窦冰然郭会明朱曼利
Owner NANJING UNIV OF TECH