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A kind of Paenibacillus and its application in water purification

A technology of Paenibacillus and Bacillus, applied to Paenibacillus and its application field, can solve the problems of large workload and high cost, and achieve the effects of strong adaptability, good microbial growth, effective and stable treatment

Active Publication Date: 2019-08-13
重庆华悦生态环境工程研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the microbial preparation depends on the composition of various microorganisms to function, and it needs to separate, screen and enrich the above-mentioned microorganisms for preparation, which requires a large workload and high cost.

Method used

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  • A kind of Paenibacillus and its application in water purification
  • A kind of Paenibacillus and its application in water purification
  • A kind of Paenibacillus and its application in water purification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Screening and identification of strains

[0038] 1. Screening

[0039] 1) Add 0.3 g of yeast powder to 100 mL of the spore sample containing the bacillus spores, gently shake at a 30°C shaker at 100 rpm, and culture for 1 week, and use a microscope to determine the growth of bacteria; the mud sample is taken from Longjiang River, Guangxi. Bacteria grow to measure the absorbance value of the bacterial culture solution at 600nm, if the value of OD600 obtained is between 0.6 and 0.8.

[0040] 2) Use membrane filtration to filter the bacteria in the above-mentioned culture solution, and wash the bacteria with 20 mL of sterile water. The inoculation amount was 5% in multiple 100mL enriched media, cultured at 45°C, and a non-inoculation control (CK) was set. Observe the growth of bacteria after culturing on a 100rpm shaker for two weeks.

[0041] 3) Dilute the enrichment medium gradually by 1, 2, 3, 4, 5 (corresponding to 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 ), respectiv...

Embodiment 2

[0050] Example 2 Purification of water

[0051] 1. Pass the typical black and smelly water body (the black and smelly water body of the Lashan River in Jinan) into a biological oxidation reactor, which is filled with commercially available silicon-based porous fillers.

[0052] The wastewater quality is as follows: pH7.0; chemical oxygen demand (COD), 45mg / L; total nitrogen (TN), 15mg / L, total phosphorus (P), 3mg / L.

[0053] 2. Inoculate the identified spore-like bacteria in the enriched medium and culture it to a concentration of 10 in a shaker at 25°C and 100 rpm. 8 Pcs / mL; then inoculate the bacterial solution (also called inocula, the same as the inoculation in the content of the invention) into the bio-oxidation reactor, the inoculation amount is 3L / m 3 . The bottom of the biological oxidation reactor is filled with air, and the amount of aeration varies according to the water quality, and the hydraulic stays for 1 hour;

[0054] 3. The power supply of the aeration equipment is a...

Embodiment 3

[0058] Example 3 Purification of water at low temperature

[0059] 1. Pass a typical black and smelly water body (the black and smelly water body of the Lashan River in Jinan) into a biological oxidation reactor, which is filled with commercially available silicon-based porous fillers. The wastewater quality is as follows: pH 7.0; chemical oxygen demand ( COD), 45mg / L; total nitrogen (TN), 15mg / L, total phosphorus (P), 3mg / L.

[0060] 2. Inoculate the identified spore-like bacteria in the enriched medium, and cultivate to a concentration of 10 in a shaker at 5°C and 100 rpm. 8 Pcs / mL; then inoculate the bacterial solution (also known as the bacterial agent, the same as the bacterial agent described in the content of the invention) to a low temperature environment ( 3 . The bottom of the biological oxidation reactor is filled with air, and the amount of aeration varies according to the water quality, and the hydraulic stays for 1 hour;

[0061] 3. The power supply of the aeration equ...

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Abstract

The invention relates to microorganisms for purifying water bodies, and specifically discloses bacillus genus YE-1 and its application. The Paenibacillus YE‑1 was deposited in the General Microbiology Center of the China Committee for the Collection of Microorganisms, with a preservation date of August 4, 2016 and a preservation number of CGMCC No.12814. Utilizing the Paenibacillus YE‑1 described in the present invention can effectively absorb and degrade organic matter in black and odorous water bodies in a biological oxidation reactor to purify water quality. Further, the distributed photovoltaic system supplies electric energy to the aeration facility of the biological oxidation reactor, the process flow does not need to provide additional electric energy, the operation cost is low, and the treatment is effective and stable.

Description

Technical field [0001] The invention relates to water purification microorganisms, in particular to a bacillus-like bacillus and its application. Background technique [0002] With the rapid economic development and acceleration of urbanization, the problems of water pollution and ecological degradation in many urban rivers in our country are very prominent, and even seasonal and perennial black and odorous water bodies have appeared. Existing black and odorous water treatment methods mainly adopt plant purification and aeration treatment, with low purification load, and the treatment process requires additional power input and high operating costs. [0003] Bacillus is the dominant species in the soil. It can strongly decompose carbon, nitrogen, phosphorus, and sulfur pollutants, and can decompose proteins and complex polysaccharides. It also plays an important role in the decomposition of water-soluble organic matter. Harmful algae and aquatic pathogens in the environment compet...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C02F2101/30C12N1/205C12R2001/01
Inventor 任南琪杨平叶文华刘兴宇
Owner 重庆华悦生态环境工程研究院有限公司
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