Method for determination of fat-soluble vitamins by liquid chromatography with semi-automatic sample processing

A fat-soluble vitamin and sample processing liquid technology, applied in the field of vitamin detection, can solve the problems of confusion in the use status of vitamin products, and achieve the effects of being suitable for clinical popularization and application, high sensitivity and reliable results.

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A fat-soluble vitamin and sample processing liquid technology, applied in the field of vitamin detection, can solve the problems of confusion in the use status of vitamin products, and achieve the effects of being suitable for clinical popularization and application, high sensitivity and reliable results.

CN106404947BActive Publication Date: 2019-04-30济南国益生物科技有限公司

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Embodiment 1

[0041] Example 1 Simultaneous Determination of Multiple Fat-soluble Vitamins in Serum Using Semi-automatic Sample Processing Liquid Chromatography Technology

[0042] Proceed as follows:

[0043] (1) Prepare the sample to be tested: put 200ul serum sample in a semi-automatic liquid-liquid extraction apparatus, first add an equal volume of frozen ethanol, vortex for 2 minutes, and then add 800ul frozen 0.4g / L 2,6-di-tert-butyl The n-hexane solution of 4-methylphenol was vortexed at 1500rpm for 10 minutes, then centrifuged at 3000rpm for 20 minutes, the supernatant was taken, and 800ul of frozen 0.4g / L 2,6-di-tert-butyl -N-hexane solution of 4-methylphenol, vortexed at 1500rpm for 10 minutes, then centrifuged at 3000rpm for 20 minutes, combined the supernatant, blown up to nearly dry with nitrogen flow, then reconstituted with 100ul frozen ethanol, vortexed for 60s, filtered through 0.2um Membrane, injection analysis. In this embodiment, two samples of sample 1 and sample 2 we...

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Abstract

The invention discloses a method for simultaneous determination of various fat-soluble vitamins in serum by using a semi-automatic sample treatment liquid chromatographic technology; the HPLC method is adopted, and the chromatographic conditions comprise that a mobile phase A is a 0.05% trifluoroacetic acid aqueous solution, a mobile phase B is a 0.02% trifluoroacetic acid methanol-ethanol mixed solution, and gradient elution is adopted; the various fat-soluble vitamins are selected from vitamin A, vitamin D2, vitamin D3, alpha-tocopherol, beta-tocopherol, gamma-tocopherol, delta-tocopherol, K1, K2 or Q10. Through optimization of a sample pretreatment method and high performance liquid chromatographic conditions, the method for simultaneous determination of the various fat-soluble vitamins in the serum is established, can carry out precise qualitative and quantitative analysis, is a detection method having the advantages of simple and fast sample processing, high flux, reliable result and low cost, has the advantages of strong specificity, high sensitivity, simple operation, low cost and the like, has easily analyzed and objective results, and is especially suitable for clinical popularization and application.

Description

technical field [0001] The invention relates to a method for simultaneously determining multiple fat-soluble vitamins in serum by using semi-automatic sample processing liquid chromatography technology, and belongs to the technical field of vitamin detection. Background technique [0002] Vitamins are a class of low-molecular compounds necessary for the body to maintain normal metabolism and functions. They are one of the seven major nutritional elements of the human body. They are divided into fat-soluble and water-soluble vitamins. Vitamins in the human body must be kept in an appropriate range to be healthy for the human body. Beneficial, whether it is lacking or increasing, it will bring harm to the human body. Vitamins A, D2, D3, alpha-tocopherol, beta-tocopherol, gamma-tocopherol, delta-tocopherol, K1, K2 and Q 10 They are all fat-soluble vitamins. When the human body lacks these vitamins, it will show different symptoms: A deficiency can easily cause night blindness,...

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Application Information

Patent Timeline

30 Apr 2019

Publication

CN106404947B

IPC: G01N30/02; G01N30/14

CPC: G01N30/02; G01N30/14; G01N2030/062; G01N2030/146

Inventors: 陈大为