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Wild bletilla striata tissue culture rapid propagation method

A tissue culture, Bletilla striata technology, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve the problems of difficult seed reproduction, incomplete embryo development, slow reproduction speed, etc., and shorten the cultivation and reproduction time and cost. Low and simple cultivation method

Inactive Publication Date: 2017-02-22
云南金碧制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The seeds of Bletilla striata are small, powder-like, without endosperm, and incompletely developed embryos are not easy to germinate, and it is difficult to carry out seed reproduction; when using division propagation, the reproduction rate is low and the reproduction speed is slow

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The described wild bletilla striata tissue culture rapid propagation method, its operation steps are:

[0025] a. Explant material and treatment, take the pseudobulb of Bletilla striata, use a knife and toothbrush to deal with the surface sundries and soil, soak it in saturated washing powder aqueous solution for 10 minutes, shake it fully and rinse it under tap water for 1-2 Hours, placed on the ultra-clean workbench to be processed;

[0026] b. Place the pseudophosphorous stem treated in step a in an ultra-clean workbench, rinse it once with sterile water, disinfect it with 75% ethanol for about 30 seconds, and rinse it once with sterile water; After 10 minutes, rinse with sterile water for 3-4 times, then cut the sterilized pseudobulbs to a size of 0.5 square centimeters, each with bud eyes, to obtain bud blocks;

[0027] c. Place the bud pieces cut in step b in the bottled callus induction medium, inoculate 3-4 pieces of the same medium in each bottle, and callus c...

Embodiment 2

[0037] Wild bletilla striata tissue culture rapid propagation method, its operation steps are:

[0038] a. Explant material and treatment, take the pseudobulb of Bletilla striata, use a knife and toothbrush to deal with the surface sundries and soil, soak it in saturated washing powder aqueous solution for 10 minutes, shake it fully and rinse it under tap water for 1-2 Hours, placed on the ultra-clean workbench to be processed;

[0039]b. Place the pseudophosphorous stem treated in step a in an ultra-clean workbench, rinse it once with sterile water, disinfect it with 75% ethanol for about 30 seconds, and rinse it once with sterile water; After 10 minutes, rinse with sterile water for 3-4 times, then cut the sterilized pseudobulbs to a size of 0.5 square centimeters, each with bud eyes, to obtain bud blocks;

[0040] c. Place the bud pieces cut in step b in the bottled callus induction medium, inoculate 3-4 pieces of the same medium in each bottle, and callus can grow after 2...

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PUM

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Abstract

The invention provides a wild bletilla striata tissue culture rapid propagation method and relates to a plant culture and propagation method, in particular to a medicine culture and propagation method. Pseudo-bulbs of purple-flower bletilla striata are used for tissue culture, the differentiation rate of the pseudo-bulbs is greatly increased and can reach 500-900% after the seudo-bulbs are cultured by a culture medium so as to adapt to quick provenance enlarging, and the transplanting survival rate can reach 98% after seedling recovering and seedling hardening. The culture method is simple and applicable, cost is low, the differentiation rate of tissue culture is increased, cultured seedlings are strong, the transplanting survival rate is high, basic differentiation is finished within one month, seedling raising can be finished 65-80 days after root inducing, seedling recovering and seedling hardening, bletilla striata culture and propagation time is greatly shortened, and the possibility of large-scale planting is provided for wild bletilla striata training.

Description

technical field [0001] The invention relates to a method for cultivating and propagating plants, in particular to a method for cultivating and propagating medicines. Background technique [0002] Bletilla striata (Thunb.) Reichb.f. is a perennial herbaceous plant of the genus Bletilla in Orchidaceae. It is widely used for beauty, whitening, freckle removal and ornamental value, and its wild resources are on the verge of extinction. [0003] The seeds of Bletilla striata are small, powder-like, without endosperm, and incompletely developed embryos are not easy to germinate, and it is difficult to carry out seed reproduction; when using division propagation, the reproduction rate is low and the reproduction speed is slow. Contents of the invention [0004] The purpose of the present invention is to overcome the defects of the prior art, and to provide a method for rapid propagation of wild bletilla striata through tissue culture with high reproduction rate and fast speed. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
CPCA01H4/008
Inventor 陈蕾
Owner 云南金碧制药有限公司