Interface device for micro-flow electrokinetic chromatography coupled with nano-liter electrospray ion source mass spectrometry

Abstract

The invention provides a connector device for combining a microflow electric chromatographic instrument and a nanoliter stage electron spray ion source mass spectrum. The connector device comprises a bracket, a connector unit, a spray needle and an electron spray electrification unit, wherein an insulation element is arranged between the connector unit and the bracket; a capillary-column chromatography connecting opening is formed in one end surface of the connector unit; a spray needle connecting opening is formed in the other end surface of the connector unit; a spray needle opening is formed in one end surface of the electron spray electrification unit; a metal plating layer is formed on the outer wall of a sample outlet end of the spray needle. The connector device provided by the invention has the advantages that the connection use of the microflow liquid phase electric chromatographic instrument and the nanoliter stage electron spray ion source mass spectrum can be realized; the analysis efficiency of ionogenic substances in a flowing phase can be effectively improved; the column efficiency is greatly improved; a flow supplementation device is not needed; meanwhile, the device has the advantages of good spraying effect, high ionization efficiency, high signal intensity, high stability and the like.

Description

technical field [0001] The invention belongs to the technical field of detection instruments, and relates to an interface device for a micro-flow liquid-phase electrochromatograph coupled with an electrospray ion source mass spectrometer, in particular to a micro-flow liquid-phase electrochromatograph coupled with a nano-level electrospray ion source mass spectrometer. Interface device with (pCEC-NanoESI-MS). Background technique [0002] In recent years, a large number of combined capillary electrophoresis-mass spectrometry (Capillary electrophoresis massspectrometry, CE-MS) techniques have been reported, and have been practically applied in many laboratory analysis and detection techniques. However, there are still many problems in the actual use of capillary electrophoresis-mass spectrometry in the prior art, so the improvement of capillary electrophoresis-mass spectrometry has become a research hotspot in this field in recent years. [0003] The combination of microflow...

AI Technical Summary

Problems solved by technology

[0004] At present, the interface design of the combination of micro-flow liquid chromatography and mass spectrometer nano-upgraded electrospray ion source mainly adopts a supplementary flow device. The main disadvantage of this design is that it is easy to cause instability of the spray effect, resulting in a decrease in sample concentration and detection sensitivity. At the same time, the supplementary flow device greatly increases the difficulty of processing. Compared with the interface device without supplementary flow device, it requires higher machining accuracy, resulting in a great increase in processing costs.

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