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Immune composition, vaccine comprising same, and applications of immune composition and vaccine

An immune composition and composition technology, applied in the field of immunology, can solve the problems of insufficiently improving the immunogenicity of weak antigens, troublesome preparation and use, and large side effects, and achieve high-level immune effects, simple use, and strong local stimulation Effect

Pending Publication Date: 2017-03-01
LUOYANG HUIZHONG ANIMAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] By applying the extract of Acanthopanax senticosus to the immune composition, it overcomes the chemical immune adjuvants such as oil emulsions and aluminum gels that are widely used at home and abroad, which often have large side effects, strong local irritation, carcinogenicity, and troublesome preparation and use. Or can not sufficiently improve the immunogenicity of weak antigens and other disadvantages

Method used

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  • Immune composition, vaccine comprising same, and applications of immune composition and vaccine
  • Immune composition, vaccine comprising same, and applications of immune composition and vaccine
  • Immune composition, vaccine comprising same, and applications of immune composition and vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-8

[0024] 1. Test material

[0025] Carbomer; 0.01mol / L PBS solution.

[0026] Xinliu dual inactivated vaccine (i.e. chicken Newcastle disease and avian influenza dual inactivated vaccine): Provided by Pulaike Bioengineering Co., Ltd.

[0027] ND standard antigen was purchased from China Veterinary Drug Administration (batch number: 201411).

[0028] Newcastle disease virus (NDV) hemagglutination inhibition test (HI) positive serum was purchased from China Veterinary Drug Control Institute (batch number: 201410).

[0029] 20×PBS, purchased from Shanghai Bioengineering Co., Ltd. (batch number: 140119081Z).

[0030] Chicken avian influenza virus (Avian influenza virus, AIV) standard antigen (H9) was purchased from Harbin Veken Biotechnology Development Company (batch number: 201401).

[0031] Avian influenza virus subtype H9 positive serum was purchased from Harbin Veken Biotechnology Development Company, production batch number: 201401.

[0032] 90 1-day-old 817 broilers.

[...

Embodiment 9-16

[0087] 1. Test material

[0088] Acanthopanax extract and Radix Astragali extract were prepared according to the method of Example 1-8, and passed the inspection.

[0089] Carbomer; 0.01mol / L PBS solution.

[0090] About 5 weeks old, healthy female clean-grade Balb / c mice were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. (with Beijing Municipal Laboratory Animal Quality Certificate).

[0091] Mouse Serum PCV2 ELISA Antibody Kit.

[0092] 2. Preparation of porcine circovirus vaccine

[0093] 2.1 Preparation of bacteria (poison) species for production

[0094] Preparation of porcine circovirus type 2 (Porcine circovirus type 2, PCV2) SH virus strain: the virus seed is diluted 10 times with DMEM medium (Invitrogen company), inoculated in PK15 (ATCC, preservation number is CCL) by 5% volume -33) Cells, adsorbed at 37°C for 30 minutes, added DMEM cell maintenance solution containing 4% calf serum and 2mmol / L D-glucosamine hydrochloride, harveste...

Embodiment 17-21

[0125] Effects of Different Immunization Compositions on Pig Immune Stress

[0126] The immune vaccine is the vaccine prepared in Examples 9-16.

[0127] 1. Piglet feeding and management

[0128] Before the test, fully sterilize, feed the test piglets in the nursery house, keep the indoor temperature at about 28°C, eat freely, drink water freely, keep the house clean, disinfect twice a week, and officially enter the test stage after 7 days of pre-test During the experiment, carefully observe and record various clinical symptoms of piglets every day, such as body temperature, feed intake, mental status and various diseases, and feed the nursery pigs full-price feed.

[0129] 2. Experimental design and grouping

[0130] According to the results of Examples 9-16, Examples 11, 12, 14, 15 and 16 were selected for the effect of the immune composition on pig immune stress.

[0131] With 70 healthy piglets of 14 days old PCV2 ELISA antibody negative, PCV2 antigen negative and PRRSV...

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Abstract

The invention relates to the technical field of immunology, and provides an immune composition, a vaccine comprising the immune composition, and applications of the immune composition and the vaccine. The composition comprises acanthopanax root extract, an avirulent antigen and a pharmaceutically acceptable carrier. The composition provided by the invention can be directly mixed with the antigen and astragalus extract randomly selected to prepare the vaccine composition, the use is simple, and the high-level immune effect can be continuously generated.

Description

technical field [0001] The invention relates to the technical field of immunology, in particular to an immune composition and a vaccine comprising the immune composition. Background technique [0002] At present, for oily adjuvanted vaccines, it is known that not only the immunopotentiating effect of such vaccines, but also the stability of the vaccine is considerably affected by the type of emulsion, the oily component used and the surface to which it is bound. The influence of factors such as the type of active agent. Especially for inactivated vaccines, it is very difficult to prepare a vaccine that can induce sufficient immune protection over a long period of time and has good reproducibility. [0003] Vaccine adjuvants are used for two purposes: first, to slow the release of antigens from the injection site, and second, to stimulate the immune system. Traditional vaccines usually consist of crude preparations of inactivated or killed or modified live pathogenic microo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/145A61K39/17A61P31/14A61P31/16
Inventor 张许科刘兴金潘贵珍
Owner LUOYANG HUIZHONG ANIMAL MEDICINE