Device and method for headspace solid phase microextraction capable of removing water

Abstract

The invention provides a device and a method for headspace solid phase microextraction capable of removing water. The device comprises a material container, a steam container, a heating type magnetic stirrer, a needle head, extraction fiber and a magnetic stirring bar, wherein the material container is a sealed container and is arranged on the heating type magnetic stirrer; the interior of the material container is filled with a to-be-extracted material and the magnetic stirring bar; the steam container is in sealed arrangement inside the material container; the interior of the steam container is filled with a water vapor absorption material; one end of the steam container is opened; the position of the wall of the material container facing the opened end of the steam container is provided with a puncturable soft plug; the needle head penetrates through the soft plug and reaches the interior of the steam container; and an extraction coating layer is arranged on the extraction fiber which extends into the steam container through the inner channel of the needle head. According to the invention, the water vapor located at the headspace part of an extraction container during headspace extraction operation can be removed through the arrangement of a simple device.

Description

technical field [0001] The invention relates to the construction field of HS-SPME devices in sample pretreatment, in particular to a device and method for water-removable headspace solid-phase microextraction. Background technique [0002] Solid Phase Microextraction (Solid Phase Microextraction, SPM E) is a sample processing method that has appeared since the 1990s. It overcomes almost all the shortcomings of some traditional sample processing before. It integrates sampling, extraction, concentration and sample injection, and can be used in conjunction with gas or liquid chromatography. As a kind of SPME, HS-SPME (Headspace Solid Phase Microextraction), on the one hand, inherits the advantages of headspace technology, which is easy to operate and not interfered by the sample matrix; on the other hand, it can concentrate while sampling, which greatly improves the Analytical sensitivity. [0003] The high temperature required for the volatilization of organic compounds in t...

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Problems solved by technology

[0003] The high temperature required for the volatilization of organic compounds in the headspace extraction process will, on the one hand, speed up the molecular motion, resulting in an increase in the vapor pressure of the liquid, which is conducive to adsorption; The content of water vapor in the medium also increases, and the content of the substance to be extracted in the headspace will decrease, which will affect the adsorption of the extraction head to the target compound, and the extraction capacity will decrease instead, which will directly affect the sensitivity and detection limit of the method.

In addition, the water vapor generated during the heating process will coexist with the vapor of the analyte in the headspace of the serum bottle, and the water vapor attached to the fiber will cause damage to the chromatographic column and mass spectrometer after entering the detection instrument, for example, Reduce the resolution of the chromatographic column and reduce the service life of the mass spectrometer detector

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