Separation Method of Capillary Size Exclusion Chromatography with Controlled Electric Field

Abstract

The invention discloses an electric field controllable capillary tube size-exclusion chromatography separation method. In the method, carbon nanofiber / quartz capillary tube or stainless steel tube is adopted as a separation column; two electrodes are placed at the two ends of the separation column while an alternating-current electric field is applied; the head of the carbon nanofiber in the capillary tube carries charges and moves up and down in the separation column under the effect of the alternating-current electric field; the pores and gaps formed by the carbon nanofiber are controlled by regulating the parameters such as voltage, frequency and Duty value of the applied electric field; and longitudinal and transverse separation channels different in size are formed in the separation column. Relatively large micro-scale substances entering the system cannot enter the pores or gaps and thus are excluded first, and relatively small micro-scale substances enter the pores and gaps and then are eluted so as to realize efficient separation of micro-scale substances different in size; and the method has the advantages of no replacement of stationary phase, wide application range, low solvent consumption and greenness and safety.

Description

technical field [0001] The invention relates to a method for controlling the size of the stationary phase in a chromatographic column through an alternating electric field, that is, an electric field controllable capillary size exclusion chromatographic separation method, specifically refers to the ability to change the modified carbon nanometer in the chromatographic column according to the alternating electric field The size of the pores and gaps formed by the fibers, thus realizing a method of separating various micro-scale substances. Background technique [0002] At present, people pay more and more attention to the separation of micro-scale substances such as cells and proteins. Therefore, various separation methods have emerged. Size exclusion chromatography, as one of the commonly used separation methods, is mainly used for large-scale Molecular separation analysis. This method is different from other chromatography separation principles, because it does not rely on...

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Problems solved by technology

[0003] The purpose of the present invention is to overcome the shortcomings of the existing size exclusion chromatography, such as high requirements for fillers, large solvent consumption, and the inability to simultaneously and efficiently separate micro-scale substances with a relative molecular weight difference within 10%. Separation Method of Capillary Size Exclusion Chromatography with Controlled Electric Field

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