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Method for adding Arthrobacter sp. LHM 7719 to ferment to produce catalase

A technology of catalase and hydrogen peroxide, which is applied in the field of fermentation to produce catalase, can solve the problems of high cost and low enzyme production by microbial fermentation, and achieve the effects of simple operation, low price and increased production

Inactive Publication Date: 2017-03-22
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, domestic and foreign catalase market demand is increasing day by day, yet the low enzyme yield of microbial fermentation method leading to higher cost is still the main problem to be solved urgently in the process of catalase industrialized production; the applicant The authorized invention patent "ZL201310505522.2 A high-yield alkaline catalase bacteria and the fermentation production process of the strain"

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Pick a single colony from the slant, streak it on the LB plate medium, put the plate upside down in a 30°C incubator and cultivate it for 24 hours, then scrape a ring of bacteria from the plate, and inoculate it into a 250mL triangle containing 40mL seed medium cultured in bottles. Culture conditions: pH 7.2, culture time 16 hours, temperature 30°C, shaking table speed 160r / min, then inoculate the cultured seed solution with 3.5% inoculation amount into a 7L fermenter containing 5L enzyme-producing medium for fermentation Enzyme test. Addition of H during enzyme-producing fermentation 2 o 2 The method is: 18 hours, 22 hours, 26 hours, 30 hours, 34 hours, 38 hours, and 42 hours after the start of fermentation, add hydrogen peroxide with a volume concentration of 0.55% in the medium in batches. Fermentation conditions: pH is 7.2, culture temperature is 30°C, fermentation time is 46 hours, rotation speed is 400r / min, air flow is 1.7L / min, the enzyme activity of the ferm...

Embodiment 2

[0032] Pick a single colony from the slant, streak it on the LB plate medium, put the plate upside down in a 30°C incubator and cultivate it for 24 hours, then scrape a ring of bacteria from the plate, and inoculate it into a 250mL triangle containing 40mL seed medium cultured in bottles. Culture conditions: pH 7.2, culture time 16 hours, temperature 30°C, shaking table speed 160r / min, then inoculate the cultured seed solution with 3.5% inoculation amount into a 7L fermenter containing 5L enzyme-producing medium for fermentation Enzyme test. Addition of H during enzyme-producing fermentation 2 o 2 The way of adding hydrogen peroxide in the fermentation process is: 18 hours, 22 hours, 26 hours, 30 hours, 34 hours, and 38 hours after the start of fermentation, add hydrogen peroxide with a volume concentration of 0.55% in batches. Hydrogen; 42 hours to 52 hours constant flow of hydrogen peroxide, so that the volume concentration of hydrogen peroxide in the medium during this p...

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Abstract

The invention discloses a method for adding Arthrobacter sp. LHM 7719 to ferment to produce catalase, and belongs to the technical field of microbial fermentation enzyme production. According to the method disclosed by the invention, the Arthrobacter sp. LHM 7719 capable of realizing a high yield of catalase is taken as a production strain, the Arthrobacter sp. LHM 7719 produces catalase after seed culture and a step of adding hydrogen peroxide with a volume ratio of 0.4%(v / v)-0.9%(v / v) in an enzyme production culture medium as an inducer, and the activity of the catalase can be obviously increased by about 1.15 times through submerged fermentation. The yield of the catalase in a 7L fermentation tank is increased to 51870U / mL from 45100U / mL before adding the Arthrobacter sp. LHM 7719, equivalently, the yield of the catalase is increased by about 15%, and the Arthrobacter sp. LHM 7719 is suitable for industrialization; and the produced catalase can be used in the fields of textile printing and dyeing industry, food industry, industrial wastewater treatment, paper making and the like.

Description

technical field [0001] The invention is a hydrogen peroxide stress Arthrobacter Arthrobacter sp. Method for producing catalase by fermentation of LHM 7719. By studying hydrogen peroxide on Arthrobacter sp. The stress effect of LHM 7719 enzyme production achieves the purpose of high catalase production, and belongs to the technical field of microbial fermentation enzyme production. Background technique [0002] Hydrogen peroxide (H 2 o 2 ), commonly known as hydrogen peroxide, is a strong oxidizing agent, mainly used in chemical synthesis, pulp bleaching, printing and dyeing, metallurgy, semiconductor material processing, and food production and processing. In 2006, the amount of hydrogen peroxide consumed in the world was close to 2.2 million tons; but the residue of hydrogen peroxide in the textile and paper industry will greatly affect the printing and dyeing effect; if the industrial wastewater of hydrogen peroxide is not removed in time, it will cause external envir...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/08C12R1/06
CPCC12N9/0065C12N1/20C12Y111/01006
Inventor 刘焕民宋勇殷树昌张峰史逸俊
Owner CHANGZHOU UNIV
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