Latcripin-8 gene fragment of mushroom C91-3 strain, and coded protein, preparation method and application thereof

Abstract

The invention discloses a Latcripin-8 gene fragment of a mushroom C91-3 strain, and a coded protein, a preparation method and application thereof. The nucleotide sequence of the Latcripin-8 gene fragment is as shown in SEQ ID No. 1, and the amino acid sequence of the coded protein is as shown in SEQ ID No. 2. The preparation method for the Latcripin-8 gene fragment comprises the following steps: extracting total RNAs of mycelia of the mushroom C91-3 strain; subjecting the total RNAs of the mycelia to inverse transcription so as to synthesize cDNAs; carrying out PCR amplification on a target gene: carrying out a PCR reaction on with the obtained cDNAs as a template and with a PCR reaction primer having two enzyme sites, i.e., BamHI and NotI; and recovering and purifying the obtained DNA fragment.

Description

technical field

[0001] The invention relates to a Latcripin-8 gene fragment extracted from Lentinus edodes C91-3 strain, a method for preparing the encoded protein and its use. The encoded protein has the functions of inducing tumor cell apoptosis, regulating body immunity, regulating cell cycle, anti-tumor and It has the functions of scavenging free radicals and decolorizing dye wastewater. Background technique

[0002] Fungi are rich in a variety of bioactive molecules, including ribosome-inactivating proteins, antifungal proteins, lectins, ubiquitin-like proteins, polysaccharides, and kinases. The anti-tumor components are unique in the treatment of tumors with the advantages of less adverse reactions and significant curative effects. Lentinus edodes fungus C91-3 belongs to Basidiomycotina Basidiomycetes Pleurotaceae, which has been preserved in General Microbiology Center of China Committee for Microbial Culture Collection in 2013 with the preservation number CGMCC No.7...

Images