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Method for enzymatic hydrolysis of dried porcine solubles from animal small intestines

A technology of intestinal membrane protein powder and enzymatic hydrolysis, which is applied in the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problem of high salt content of intestinal membrane protein powder, poor quality of intestinal membrane protein powder, intestinal membrane protein The powder is easy to be corrupted and other problems, and achieves the effect of good bioavailability, reasonable steps and bright yellow color

Inactive Publication Date: 2017-03-22
广州市嘉仁高新科技有限公司
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Problems solved by technology

[0002] Now two methods are widely used in the market to produce intestinal membrane protein powder, one is the salt hydrolysis method, extracting the residue of heparin sodium, and using the residue to produce intestinal membrane protein powder after enzymatic hydrolysis, the intestinal membrane protein powder produced by this method The quality is poor, the salt content is high, it is easy to deteriorate, and it is not suitable for long-distance transportation, and the intestinal membrane protein powder produced by this method is easy to be corrupted during the drying process, and it will bring in sediment when it is naturally dried; the other is the enzymatic hydrolysis method. It can produce intestinal membrane protein powder while producing heparin sodium, but the intestinal mucosa used in the current enzymatic hydrolysis method is generally processed intestinal mucosal powder, and there is no desalination step in the operation process of some enzymatic hydrolysis methods, so that The salt content in the prepared intestinal membrane protein powder is too high

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  • Method for enzymatic hydrolysis of dried porcine solubles from animal small intestines

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Embodiment Construction

[0021] Fresh pig small intestine is taken as a raw material, and intestinal membrane protein powder is prepared according to the method for producing enzymatic intestinal membrane protein powder from animal small intestine. The method comprises the following steps:

[0022] (1) Intestinal mucosa pretreatment, using an intestinal scraper to scrape and separate the intestinal mucosa from the small intestine of the animal, use a container to collect the intestinal mucosa, add an aqueous solution containing 2.5% NaCl to the container to clean the intestinal mucosa, and filter it after cleaning to obtain clean intestinal mucosa;

[0023] (2) Enzymolysis, put the clean intestinal mucosa into the enzymolysis container, add water to the enzymolysis container, after adding water, use a stirring device to fully stir at a temperature of 40-50°C, and a stirring speed of 300- 600r / min, the stirring time is 15-30min, then use the stirring device to fully stir, then add protease to the enzym...

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Abstract

The invention discloses a method for enzymatic hydrolysis of dried porcine solubles from animal small intestines, and relates to the field of the production of the dried porcine solubles. The method comprises the following steps: (1) pre-processing intestinal mucosa; (2) conducting enzymatic hydrolysis; (3) conducting fine filtration; (4) conducting desalting; (5) conducting concentrating; and (6) conducting spray-drying. The method has the advantages of being reasonable in steps and convenient to operate, the prepared dried porcine solubles are good in quality, high in digestion rate and good in bioavailability, a salt content of the dried porcine solubles is less than 5% and an ash content is less than 10%, the dried porcine solubles are presented in bright yellow, the dried porcine solubles are aromatic in smell and salty and delicious in taste, without hit to teeth, and harmful pathogenic microorganisms can be killed in a production process, so that biological safety is enhanced.

Description

technical field [0001] The invention relates to the production field of intestinal membrane protein powder, in particular to a method for producing enzymatically hydrolyzed intestinal membrane protein powder from animal small intestine. Background technique [0002] Now two methods are widely used in the market to produce intestinal membrane protein powder, one is the salt hydrolysis method, extracting the residue of heparin sodium, and using the residue to produce intestinal membrane protein powder after enzymatic hydrolysis, the intestinal membrane protein powder produced by this method The quality is poor, the salt content is high, it is easy to deteriorate, and it is not suitable for long-distance transportation, and the intestinal membrane protein powder produced by this method is easy to be corrupted during the drying process, and it will bring in sediment when it is naturally dried; the other is the enzymatic hydrolysis method. It can produce intestinal membrane prote...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34
Inventor 宗志伦
Owner 广州市嘉仁高新科技有限公司
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