Kit and method for gene chip detection of 16 respiratory pathogens

Abstract

The invention discloses a kit and a method for gene chip detection of 16 respiratory pathogens. The kit comprises a PCR buffer solution, a reverse transcriptase, dNTPs, primers, probes, a Taq polymerase and metal cations, wherein the probes comprise hybridization probes and internal standard probes; the primers comprise 16 respiratory pathogen primers, 2 internal standard primers and 1 universal primer; the sequences of the primers are shown in SEQ ID NO:1-37; the sequences of the probes are shown in SEQ ID NO:38-55. On the basis of the PCR technology, the primers are designed for conserved domains of the 16 respiratory pathogens to amplify the target nucleotide sequence, an amplification product is subjected to hybridization and color development by means of a gene chip hybridization technology, color development conditions of hybridized spots on a gene chip are compared with a standard card finally, and a detection result is analyzed and judged.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a gene chip detection kit and method for detecting 16 kinds of respiratory pathogens. Background technique [0002] Respiratory tract infection is one of the most common diseases in the world, and the incidence rate occupies a major position in the overall structure of the morbidity rate of residents in various countries. About 10% of the residents suffer from respiratory tract infection in the peak period of the epidemic each year. Respiratory tract infection can cause rhinitis, runny nose, stuffy nose, cough, mild pharyngitis, general fever and other symptoms, and severe cases can lead to dyspnea and even death. According to the World Health Organization's statistical report in 2002, respiratory infection ranks third among the top ten causes of death in the world, and nearly four million people die from respiratory infection in the world every year. [0003] Gene ...

AI Technical Summary

Problems solved by technology

Although fluorescent PCR can obtain better sensitivity and specificity, due to the limitation of the signal acquisition channel of the instrument, only a few pathogens can be detected in each reaction, and the throughput is low, and it will greatly increase when multi-tube detection is adopted. The economic cost and time cost of detection; although the serum immunoassay reagent can obtain a large detection throughput, but because the serum immunoassay detects the antibodies produced in the serum, there is a long window period, and The sensitivity is relatively low; the detection of bacterial pathogens is more difficult. Although the bacterial culture method has high accuracy, the cycle is long and the culture effect is poor. Many bacteria cannot be cultured, which delays the best time for treatment.

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