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Application of SR-A as diagnostic marker and intervention target for rheumatoid arthritis

An arthritis and marker technology, applied in the field of clinical medicine, to achieve the effect of good diagnostic specificity, improved detection accuracy, and good treatment effect

Active Publication Date: 2017-03-22
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, whether SR-A can be used as a specific diagnostic marker and therapeutic target for RA has not been reported yet.

Method used

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  • Application of SR-A as diagnostic marker and intervention target for rheumatoid arthritis
  • Application of SR-A as diagnostic marker and intervention target for rheumatoid arthritis
  • Application of SR-A as diagnostic marker and intervention target for rheumatoid arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment l

[0029] Embodiment 1 SR-A is used as the sensitivity and the specificity of marker diagnosis RA

[0030] Based on enzyme-linked immunosorbent assay (i.e., ELISA method), antibodies that specifically recognize class A scavenger receptor proteins (mouse anti-human SR-A monoclonal antibody and HRP-labeled rabbit anti-human SR-A monoclonal antibody, purchased from Beijing Yiqiao Shenzhou Biotechnology Co., Ltd.) and class A scavenger receptor protein standard (Recombinant Human SR-A, American R&D company) were used as diagnostic reagents for RA, osteoarthritis, systemic lupus erythematosus, Sjogren's syndrome and SR-A in normal human serum is detected to evaluate the distribution of SR-A in various diseases and its value in diagnosing RA.

[0031] The reagent combination used to detect the concentration of SR-A protein in the subject's serum sample includes the following components: a class A scavenger receptor protein standard, a capture antibody (capture) that binds to a microtit...

Embodiment 2

[0053] The neutralizing antibody of embodiment 2SR-A inhibits the differentiation of RA monocytes to osteoclasts

[0054] Through in vitro osteoclast induction experiments, the inhibitory effect of SR-A neutralizing antibody on osteoclastogenesis was detected, and the potential value of SR-A as an RA intervention target was evaluated.

[0055] Aseptically isolate PBMC (peripheral blood mononuclear cells) from patients with rheumatoid arthritis, and spread them into 12-well cell culture plates (5×10 5 Cells / well), change the medium after two hours of adherence, keep only the adherent monocytes (osteoclast precursor cells), and add 30ng / mL M-CSF (macrophage colony-stimulating factor) and 25ng / mL ml of RANKL (receptor activator of nuclear factor kappa B ligand).

[0056] 2μg / mL anti-SR-A neutralizing antibody (Mouse anti-human SR-A Neutralization Antibody, American R&D Company) and isotype antibody (Mouse IgG1, American R&D Company) were added to two wells of cells from the same...

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Abstract

The present invention discloses application of an A-type scavenger receptor (SR-A) as a diagnostic marker and an intervention target for rheumatoid arthritis, the accuracy rate of an A-type scavenger receptor (SR-A) protein as a marker for clinical diagnosis of the rheumatoid arthritis reaches to 90%, and compared with the accuracy rate of other diagnostic markers, the detection accuracy is greatly improved. In the aspect of treatment of the rheumatoid arthritis (RA), the SR-A as a therapeutic target also shows excellent performance, a SR-A neutralizing antibody can effectively prevent development of RA disease, and especially shows a very good therapeutic effect on disabling RA bone destruction.

Description

technical field [0001] The invention relates to the technical field of clinical medicine, in particular to the application of class A scavenger receptor (SR-A) protein as a diagnostic marker and intervention target for rheumatoid arthritis. Background technique [0002] Rheumatoid arthritis (RA) is a chronic, progressive, erosive systemic autoimmune disease mainly manifested by symmetrical polyarthritis. The prevalence of rheumatoid arthritis in the world is about 1%, and the incidence rate of adults is 0.03%. In my country, the prevalence of the disease is 0.24% to 0.4%, and the number of patients is about 5 million, which is equivalent to the population of a medium-sized city. Without formal treatment, the disability rate reaches 75%. [0003] The lesions of RA are characterized by erosive synovitis and the resulting destruction of articular cartilage and bone, eventually leading to joint deformity. If the disease cannot be diagnosed and treated in time, it can develop ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/102
Inventor 栗占国胡凡磊王向阳李英妮蒋向石连杰刘洪江徐丽玲
Owner PEOPLES HOSPITAL PEKING UNIV
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