Method for induction of lycium ruthenicum murr tetraploid through tissue culture

A technology of black fruit Lycium barbarum and tissue culture, which is applied in horticultural methods, botanical equipment and methods, applications, etc., to achieve the effects of ensuring fruit quality, realizing industrialization, and meeting market demand

Inactive Publication Date: 2017-04-19
TIANJIN AGRICULTURE COLLEGE
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  • Application Information

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  • Method for induction of lycium ruthenicum murr tetraploid through tissue culture
  • Method for induction of lycium ruthenicum murr tetraploid through tissue culture
  • Method for induction of lycium ruthenicum murr tetraploid through tissue culture

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Embodiment Construction

[0019] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0020] A method utilizing tissue culture to induce tetraploid of Lycium barbarum, the steps are as follows:

[0021] The test materials for the following methods are aseptic seedlings of Lycium barbarum black fruit, obtained by tissue culture of Lycium barbarum seeds purchased in Xining, Qinghai, and are now preserved in the Plant Teaching and Research Section of the School of Horticulture and Landscape Architecture, Tianjin Agricultural University.

[0022] ⑴ Obtaining sterile vaccines

[0023] Select black fruit wolfberry seeds with consistent size and full grain, select 1200 seeds, load them into 40 tissue culture bottles respectively, take 30 grains as a group, disinfect them in 75% alcohol for 3 minutes, and wash them with sterile water...

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Abstract

The invention discloses a method for induction of lycium ruthenicum murr tetraploid through tissue culture. The method comprises the following steps that (1) aseptic seedlings are obtained, and blades are picked; and (2) the blades of lycium ruthenicum murr are used as explants of tetraploid induction to be placed on a lycium ruthenicum murr tetraploid callus induction culture medium for callus induction, specifically, the lycium ruthenicum murr tetraploid callus induction culture medium comprises 0.125 mg.L<-1> of 6-BA, 0.025 mg.L<-1> of 2,4-D and 2% of DMSO and 0.01 mg.L<-1> of colchicines. The tetraploid calluses of the lycium ruthenicum murr are successfully induced through combination of dimethyl sulfoxide with the colchicines, and the method lays a foundation for induction of lycium ruthenicum murr tetraploid plants.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, and relates to a method for inducing quadrupling of Lycium barbarum by tissue culture. Background technique [0002] Lycium ruthenicum Murr. is a perennial deciduous shrub of the genus Lycium L. in the family Solanaceae. It is often distributed in the form of shrubs in saline-alkali wasteland and sandy land at an altitude of 400-3000m. It is a salt-tolerant and drought-tolerant plant. Lycium barbarum black fruit is naturally distributed in northern Shaanxi, Ningxia, Gansu, Qinghai, Xinjiang and Tibet in China, and a small amount is distributed in the Caucasus of Central Asia and Europe. 20-150cm high, multi-branched, hard and curved branches; 2-6 leaves clustered on short branches, fleshy, strip-shaped or strip-lanceolate, about 5mm long; 1-2 flowers born on both sides of the base of spines On short branches; pedicel is thin, calyx is narrow and bell-shaped; berry is spherical, purple-bl...

Claims

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Application Information

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IPC IPC(8): A01H1/08A01H4/00
CPCA01H1/08A01H4/008
Inventor 裴毅聂江力杨雪君毛金枫徐雯
Owner TIANJIN AGRICULTURE COLLEGE
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