In vitro simulated skin model

Abstract

The invention discloses an in vitro simulated skin model. According to an establishment method of the in vitro simulated skin model, a cell culture chamber and well plates are taken as the skeleton of the in vitro simulated skin model; the cell culture chamber is inoculated with immortalized epidermis cells, and a lower chamber well plate is inoculated with fibroblast for co-culture. The establishment method is capable of simulating the basic structure of human skin effectively; the in vitro simulated skin model can be applied in evaluation of transdermal absorption and functions in promoting fibroblast proliferation and migration of cosmetics and medicinal compounds effectively, can be used for replacing conventional animal experiments and in vitro skin tissue experiments, is capable of reducing experiment cost effectively, increasing experimental flux and efficiency, and preferably satisfying requirements on scientific research ethics with more and more attention.

Description

technical field [0001] The invention belongs to the fields of biomedicine and cosmetics, and relates to the design and cosmetic application of a simulated skin model in an isolated state. Background technique [0002] The skin is located on the surface of the human body and is the largest organ of the human body that is in direct contact with the external environment. It is mainly divided into four parts: epidermis, dermis, subcutaneous tissue, and skin appendages. The epidermis is divided into stratum corneum, transparent layer, granular layer, etc.; the stratum corneum is the outermost layer of the epidermis, which is composed of 4 to 8 layers of flat, non-nucleated keratinized dead cells closely arranged. The thickness of the stratum corneum is an important determinant that affects the absorption of nutrients and beauty factors by the skin. The dermis is located under the epidermis and is firmly connected with the epidermis in a wavy shape, and its thickness is about 10...

AI Technical Summary

Problems solved by technology

However, as a biomacromolecule, poor transdermal absorption has always been a major problem plaguing its practical application.

Studies have shown that the cumulative transdermal rate of EGF is only 0.993% after being applied at a dose of 0.5 μg / g for 12 hours, which proves that most of the EGF fails to penetrate the skin. At medium time, its transdermal absorption effect is very weak

Images