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Rapid propagation method applicable to tissue culture of Dalbergia hupeana Hance

A technology of Dalbergia group and Dalbergia, which is applied in the field of plant propagation, can solve the problems of low bud multiplication coefficient and slow growth, and achieve the effect of simple operation process, large yield and excellent quality of subcultured buds

Inactive Publication Date: 2017-04-26
唐春艳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a Dalbergia twig tissue culture propagation method with good growth effect and fast propagation for the problems of low bud proliferation coefficient and slow growth in the Dalbergia tissue culture process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Selection of explants: Collect semi-lignified and vigorously growing Dalbergia twigs of the year as explants;

[0026] (2) Treatment of explants: Rinse the explants under tap water for 10 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 15 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0027] (3) Initial bud induction culture: Inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 500 lx, and culture at 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified MS medium+6-BA 4.0 mg...

Embodiment 2

[0033] (1) Selection of explants: Collect semi-lignified and vigorously growing Dalbergia twigs of the year as explants;

[0034] (2) Treatment of explants: Rinse the explants under tap water for 15 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 20 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0035] (3) Initial bud induction culture: inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 1000 lx, and culture temperature 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified MS medium+6...

Embodiment 3

[0041] (1) Selection of explants: Collect semi-lignified and vigorously growing Dalbergia twigs of the year as explants;

[0042] (2) Treatment of explants: Rinse the explants under tap water for 15 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 20 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0043] (3) Initial bud induction culture: inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 1000 lx, and culture temperature 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified MS medium+6...

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PUM

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Abstract

The invention discloses a rapid propagation method applicable to tissue culture of Dalbergia hupeana Hance. The method comprises procedures of explant selection, explant treatment, initial bud induction culture, multiplication culture, bud strengthening culture and rooting culture; semi-lignified and robustly growing annual Dalbergia hupeana Hance shoots are collected as explants, the explants are trimmed, sterilized and disinfected, an initial bud induction culture medium is inoculated with the explants, initial buds are acquired, a multiplication culture medium is inoculated with the initial buds, cluster buds are formed through 3-4 cycles of multiplication culture, a bud strengthening culture medium is inoculated with the cluster buds for culture, and finally, single buds which are higher than or equal to 2 cm are inserted into a rooting culture medium for root induction. The seedling culturing cycle of the Dalbergia hupeana Hance is shortened, the seedling culturing materials are saved, the problems that many materials are required for seedling culturing, the cycle is long and the like in a conventional seedling culturing method are solved, the production cost is greatly reduced, the production efficiency is improved, and the method has better economic benefits, social benefits and ecological benefits.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and relates to a rapid propagation method suitable for tissue culture of Dalbergia chinensis. Background technique [0002] Dalbergia hupeana Hance, Fabaceae, alias: I don't know spring, Wangshui sandalwood, sandalwood, etc., tree, 10-20 meters high, dark gray bark, peeling off in flakes, produced in Shandong, Jiangsu, Anhui, Zhejiang , Jiangxi, Fujian, Hubei, Hunan, Guangdong, Guangxi, Sichuan, Guizhou, and Yunnan, at an altitude of 600-1400 meters, can grow in plains and mountainous areas, likes light, is resistant to drought and barren, and does not choose soil, but it can be grown in deep, moist and well-drained areas The soil grows well, avoid saline-alkali soil; deep roots, strong germination, born in mountain forests or shrubs, common in mountain valleys and streams and slopes with small forests, its root bark, excavated in summer and autumn, tastes pungent and bitter, Xingping,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 唐春艳陈素云
Owner 唐春艳