Myricetin extract, medicine composition containing myricetin extract and purposes thereof

A technology of extract and myricetin, which can be used in the field of medicines, health products or food supplements to inhibit tumor cell migration, can solve unseen problems, alleviate side effects, improve extraction yield, and save extraction time

Active Publication Date: 2017-04-26
BEIJING NORMAL UNIVERSITY
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, it has been found that myricetin also has an anti-tumor effect, but so far there has been no report of using myricetin as a drug to inhibit tumor metastasis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Myricetin extract, medicine composition containing myricetin extract and purposes thereof
  • Myricetin extract, medicine composition containing myricetin extract and purposes thereof
  • Myricetin extract, medicine composition containing myricetin extract and purposes thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1: the preparation of myricetin extract of the present invention

[0075] 1. Raw materials and equipment

[0076] Black beans (produced in Heilongjiang), ethanol, petroleum ether, methanol, chloroform, silica gel;

[0077] Vacuum drying oven, centrifuge, mortar, sieving apparatus, microwave ultrasonic instrument, rotary evaporator, vacuum pump, vacuum freeze drying oven;

[0078] 2. Extraction method (take black soybean skin as an example)

[0079] 2.1. Soak and peel the black beans, wash, dry, and then crush and sieve to obtain 80-100 mesh black soybean powder. Accurately weigh 10g of black soybean skin powder, dissolve the black soybean skin powder in 150mL of petroleum ether, and reflux at 80°C to degrease 2 hours; then centrifuge at 5000rpm, 25°C for 10min, remove the supernatant, vacuum dry and pulverize to 80-100 mesh at 60°C, vacuum degree -0.07MPa;

[0080] 2.2. Dry the degreased black soybean hulls at low temperature, dissolve them in 90 mL of m...

Embodiment 2

[0088] Embodiment 2: the preparation of myricetin extract of the present invention

[0089] 1. Reagents and raw materials

[0090] Bayberry leaves (produced in Zhejiang), ethanol, petroleum ether, methanol, chloroform, silica gel; vacuum oven, centrifuge, mortar, sieve, microwave ultrasonic instrument, rotary evaporator, vacuum pump, vacuum freeze-drying box;

[0091] 2. Extraction method (taking bayberry leaves as an example)

[0092] 2.1. Crush and sieve red bayberry leaves to obtain 80-100 mesh red bayberry leaf powder. Accurately weigh 10g of red bayberry leaf powder, dissolve the red bayberry leaf powder in 150mL petroleum ether, and reflux and degrease at 80°C for 2 hours; then at 5000rpm, Centrifuge at 25°C for 10 minutes, remove the supernatant, vacuum-dry and pulverize to 80-100 mesh at 60°C and vacuum degree of -0.07MPa;

[0093] 2.2. Dry the degreased red bayberry leaves at low temperature, dissolve them in 90mL of 75% methanol aqueous solution by volume, and e...

Embodiment 3

[0101] Embodiment 3: the in vitro growth inhibition test of myricetin extract to human breast cancer brain metastases cell line (BCBM), transfection migration inhibition assay and invasion inhibition assay

[0102] 1. Materials and methods

[0103] 1.1. Reagents and raw materials

[0104] 6-well plate, Transwell chamber (pore size 8 μm), 1640 basal medium, 1640 complete medium (containing 10% fetal bovine serum), 0.25% trypsin, penicillin-streptomycin, Giemsa's Stain, MTT (5mg / mL); Human breast cancer brain metastasis cell line (BCBM).

[0105] 2. Method

[0106] 2.1 In vitro growth inhibition test (MTT method) of myricetin extract on human breast cancer brain metastases cell lines

[0107] Collect logarithmic phase BCBM cells, adjust the concentration of cell suspension to 8×10 4 Cells / mL, add 100 μL per well to plate the cells to be tested at a density of 8000 cells / well and inoculate them in a 96-well plate. 5%CO 2 , incubate at 37°C until the cells adhere to the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a myricetin extract, which is prepared by an extraction method comprising the following steps that (1) plants of myricaceae, vitaceae, leguminosae or theaceae are smashed; after degreasing, the smashed plants are dissolved in a methanol aqueous solution and are subjected to microwave and ultrasonic extraction; and an extraction solution is obtained; (2) the extraction solution obtained in the step (1) is subjected to pressure reduction concentration to obtain a concentrated solution; pressure-reduction suction filtration is performed; the solution is extracted by trichloromethane; the extraction solution is collected; and freeze drying and smashing are performed; (3) powder obtained in the step (2) is dissolved in petroleum ether and then passes through a silica gel column; purification is performed; eluate containing myricetin is collected; concentration is performed; and a myricetin crude product is obtained; and (4) the myricetin crude product obtained in the step (3) is added into the methanol aqueous solution with the volume ratio being 50 to 75 percent; heating is performed to 75 to 80 DEG C until the mixture is completely dissolved; stirring is performed; then, temperature reduction still standing, crystallization, suction filtration and drying are performed; and the myricetin extract is obtained. The myricetin extract can achieve a good effect of preventing or treating tumor metastasis after being taken for a long time, and has wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of pharmacy. Specifically, the present invention relates to the myricetin extract extracted from plants, the pharmaceutical composition containing it and its preparation for preventing and / or treating diseases caused by tumor metastasis or for inhibiting tumor cell migration. , health products or food supplements. Background technique [0002] According to the latest report of the International Cancer Research Center (IARC), cancer patients worldwide are increasing at an average annual rate of 3%-5%, and cancer has become the largest public health problem in the world. According to the report of the World Health Organization, about 80% of cancer patients worldwide died of recurrence and metastasis after surgery, radiotherapy and chemotherapy. According to data from the American Cancer Society in 2015, the overall 5-year survival rate of patients after tumor metastasis is much lower than that of primary canc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K36/87A61K36/48A61K36/82A61P35/00A61P35/04A23L33/105
CPCA23V2002/00A61K36/185A61K36/48A61K36/82A61K36/87A61K2236/333A61K2236/51A61K2236/53A61K2300/00A23V2200/308A23V2250/21A23V2250/214A23V2300/14A23V2300/48A23V2300/50A23L33/105A61P35/00A61P35/04
Inventor 韩梅慈英倩
Owner BEIJING NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap