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Monoclonal antibody of apolygus lucorum ecdysone resistant receptor protein and application thereof

A monoclonal antibody, chlorotic bug technology, applied in the direction of anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, anti-animal/human immunoglobulin, immunoglobulin, etc. Food safety, decline in drug resistance and other issues

Active Publication Date: 2017-04-26
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, chemical pesticides are mainly used to prevent and control Lygus spp., but it also brings many problems, such as the decline of control effect caused by the emergence of drug resistance, pollution of the environment and food safety, etc.

Method used

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  • Monoclonal antibody of apolygus lucorum ecdysone resistant receptor protein and application thereof
  • Monoclonal antibody of apolygus lucorum ecdysone resistant receptor protein and application thereof
  • Monoclonal antibody of apolygus lucorum ecdysone resistant receptor protein and application thereof

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Embodiment 1

[0033] Embodiment 1 Preparation of anti-AlEcR-A protein monoclonal antibody

[0034] 1. Construction of pCzn1-AlEcR-A recombinant expression plasmid

[0035] Using the method based on PAS (PCR-based Accurate Synthesis), the gene AlEcR-A was connected into the expression vector pCzn1 through the cloning sites Nde I and Xba I; the obtained recombinant plasmid pCzn1-AlEcR-A was transferred into the TOP10 clone strain; Positive clones are sequenced, and the sequencing results are compared with the expected sequence, and some sequences are intercepted for comparison. figure 1 shown; the sequencing results showed that T4 DNA was successfully connected to the expression vector pCzn1 after double enzyme digestion and the T vector containing the AlEcR-A sequence, indicating that AlEcR-A had been subcloned into the pCzn1 vector, which was named pCzn1-AlEcR- A( figure 2 ); at the same time, carry out plasmid digestion identification (AlEcR-AD260 / OD280:1.84) enzyme digestion identifica...

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PUM

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Abstract

The invention discloses a monoclonal antibody capable of being specifically bound with apolygus lucorum AlEcR-A protein. The invention also discloses a mouse hybridoma cell line with a preservation number of CCTCC NO: C2016206. The monoclonal antibody provided by the invention can be specifically bound with apolygus lucorum total protein and AlEcR-A recombinant protein. The invention also discloses an application of the monoclonal antibody in comprehensive treatment of apolygus lucorum. The invention also discloses a detection kit comprising the abovementioned monoclonal antibody.

Description

technical field [0001] The invention relates to the field of antibody engineering agricultural science and technology, in particular to a monoclonal antibody against the ecdysone receptor protein of Lygus green bug, a hybridoma cell strain producing the antibody, and the use of the monoclonal antibody. Background technique [0002] Since the large-scale commercial planting of Bt cotton in my country in the 1990s, its target Phosphoptera pests such as cotton bollworm (Helicoverpa armigera H.) have been effectively controlled, and the frequency and use of chemical pesticides in cotton fields have been greatly reduced. As a result, a series of succession changes have taken place in the ecological niche of cotton pests, and Apolygus lucorum M. has risen to become the main disaster-causing pest in the Yangtze River, Yellow River Basin and Xinjiang cotton area in my country. At present, chemical pesticides are mainly used for the prevention and control of Lygus japonica, but it als...

Claims

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Application Information

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IPC IPC(8): C07K16/28C12N5/20G01N33/68G01N33/577A01N63/02A01P7/04
CPCA01N63/10C07K16/2869
Inventor 谭永安肖留斌柏立新赵静孙洋
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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