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A Paenibacillus polymyxa km2501-1 and its application

A technology of bacillus and polymyxa, which is applied in the field of agricultural microbiology to achieve the effect of promoting growth

Active Publication Date: 2019-02-26
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The control of Haemonchus contortus mainly relies on some anthelmintics, but due to the widespread drug resistance, new drugs are urgently needed to be developed

Method used

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  • A Paenibacillus polymyxa km2501-1 and its application
  • A Paenibacillus polymyxa km2501-1 and its application
  • A Paenibacillus polymyxa km2501-1 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Obtaining of Bacillus polymyxa KM2501-1:

[0034] The applicant isolated a strain of Bacillus polymyxa KM2501-1 from the rhizosphere soil of organic compound contaminated buttercup in Hukou County, Jiangxi Province. This strain was sent to the China Type Culture Collection on December 6, 2016 for preservation and named after classification : Paenibacillus polymyxa KM2501-1, deposit number: CCTCC NO: M 2016720, address: Wuhan University, Wuhan, China. The physiological and biochemical characteristics and culture characteristics of Bacillus polymyxa KM2501-1 are shown in Table 1.

[0035] Table 1 Physiological and biochemical characteristics and culture characteristics of Bacillus polymyxa KM2501-1

[0036]

[0037]

Embodiment 2

[0039] Preparation of Bacillus polymyxa KM2501-1 fermentation broth:

[0040] Streak Bacillus polymyxa KM2501-1 on the plate for activation, take a single colony on the plate into an Erlenmeyer flask containing 100 ml of KMB medium, culture temperature 28 ℃; shaker speed 180rpm; culture time 48h; The fermentation broth of Bacillus polymyxa KM2501-1 (the present invention or KM2501-1 fermentation broth for short) was centrifuged at 8000 rpm for 15 minutes to obtain the fermentation supernatant of Bacillus polymyxa KM2501-1 (the present invention or KM2501-1 fermentation supernatant for short) ) And bacteria, resuspend the bacteria in double distilled water, and OD 600 The value was adjusted to 0.8 as the Bacillus polymyxa KM2501-1 bacterial suspension (this invention or KM2501-1 bacterial suspension for short), which was used in the following examples.

Embodiment 3

[0042] The application of Bacillus polymyxa KM2501-1 in the preparation of a medicine for treating or preventing plant root-knot nematode disease includes the following steps:

[0043] Toxicity of KM2501-1 to Meloidogyne incognita

[0044] 1) Use a 96-well plate for virulence determination, add 20-30 Meloidogyne incognita per well;

[0045] 2) Add 120 μL of filtered and sterilized Bacillus polymyxa KM2501-1 fermentation supernatant (prepared in Example 2) to each hole;

[0046] 3) 4 groups of experimental settings:

[0047] KM2501-1 fermentation supernatant group (1×CF): KM2501-1 fermentation supernatant + Meloidogyne incognita;

[0048] 1 / 2 concentration KM2501-1 fermentation supernatant group (1 / 2×CF): 1 / 2 concentration KM2501-1 fermentation supernatant + Meloidogyne incognita;

[0049] 1 / 4 concentration KM2501-1 fermentation supernatant group (1 / 4×CF): 1 / 4 concentration KM2501-1 fermentation supernatant + Meloidogyne incognita;

[0050] Control group (CK): KMB medium + Meloidogyne incog...

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Abstract

The invention discloses a Paenibacillus polymyxa KM2501‑1 and application thereof. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is: CCTCC NO: M 2016720. The volatile substances produced by the solid-state fermentation of Paenibacillus polymyxa KM2501-1 provided by the invention have a good control effect on the plant pathogenic nematode Meloidogyne incognita and the animal pathogenic nematode Haemonchus contortus; the fermentation supernatant of the bacterium It also has a good effect of preventing and controlling root-knot nematode and various pathogenic fungi; at the same time, Paenibacillus polymyxa and its metabolites have good characteristics of promoting tomato plant growth; at the same time, the fermentation supernatant has a good effect of inhibiting root growth. Knot nematode egg hatching effect; Paenibacillus polymyxa KM2501‑1 has multiple ways to control nematodes, which is safe and has a good application prospect.

Description

Technical field [0001] The invention belongs to the technical field of agricultural microbiology, and specifically relates to a Bacillus polymyxa KM2501-1 and an application thereof. Background technique [0002] Root-knot nematode disease is a worldwide distributed soil-borne disease. Root-knot nematode disease caused by root-knot nematodes is one of the serious hazards that occur on vegetables. It mainly harms many common vegetables such as cucumbers, tomatoes and eggplants. And with the adjustment of the planting industry structure and the rapid expansion of the cultivation area of ​​vegetable protected areas, the occurrence of root-knot nematode disease is serious year by year, and the yield loss reaches 30%-50%. At the same time, plant root-knot nematode damage has aggravated the occurrence of soil-borne fungal diseases such as fusarium wilt and root rot and some bacterial diseases. Has become a major obstacle to current vegetable production. Therefore, the prevention and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P5/00A01P3/00A01P21/00C12R1/01
CPCA01N63/00A01N63/10C12N1/205C12R2001/01
Inventor 张吉斌程万里聂啟玉杨景艳蔡珉敏郑龙玉喻子牛胡敏高冲
Owner HUAZHONG AGRI UNIV