Primer combination and kit for y chromosome microdeletion detection

Abstract

The invention relates to a primer combination and kit for chromosome microdeletion. By the use of a multiple PCR combined with agarose gel electrophoresis technology, seven STS loci closely associated with the Y chromosome microdeletion are detected to judge the microdeletion conditioof a Y chromosome. Seven STS loci with high specificity and sensitivity (sY84, sY86, sY127, sY134, sY254, sY255 and sY160) are chosen as detection loci, at the same time two loci ZFX / Y and SRY are utilized as quality control loci, a primer is designed aiming at each locus respectively, and products of the nine loci after multiplex amplification are directly put on the agarose gel to be conducted electrophoresis detection. By the use of the primer combination and detection system, the Y chromosome microdeletion can be detected only needing single tube amplification reaction.

Description

technical field [0001] The invention relates to the field of biotechnology detection, in particular to a primer combination and a kit for detecting Y chromosome microdeletion. Background technique [0002] It is estimated that approximately 15% of couples of reproductive age are infertile, with male factors accounting for approximately 50%. The main cause of male infertility is the man's spermatogenesis disorder, manifested as severe oligospermia (less than 2×10 6 / ml~5×10 6 / ml) or no sperm, and about 20% of clinically infertile men are caused by hereditary, non-obstructive azoospermia or oligospermia, and most of these patients have normal karyotype tests. Among the known genetic factors leading to male infertility, Y chromosome microdeletion is the second most common genetic factor after Klinefelter syndrome. [0003] In 1976, Tiepolo et al., in analyzing the karyotypes of more than 1,000 male infertility patients, found that the long arm of the Y chromosome in several...

AI Technical Summary

Problems solved by technology

In 2013, EAA and EMQN published a revised version of the guidelines for the molecular diagnosis of Y chromosome microdeletions, pointing out the method of using the above 6 STS loci to detect the deletion of the AZF region (usually two multiplex PCR reactions, each reaction to detect different regions 3 STS loci) can effectively and accurately diagnose Y chromosome microdeletions, but new detection methods that add or include more STS loci cannot improve the sensitivity of detection, and may even increase the complexity of interpretation of the results, so It is not recommended to add more STS sites

Two amplification reactions not only increase the workload and difficulty of analysis, but also increase the chance of cross-contamination due to sample confusion or other reasons

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