Screening method of anti myocardial ischemia active components of flower of Polygonum orientale

An anti-myocardial ischemia, active ingredient technology, applied in material separation, analysis materials, measuring devices, etc., can solve the problem of unclear pharmacodynamic material basis, and achieve the effect of eliminating the interference of a large number of non-active components
CN106596807AActive Publication Date: 2017-04-26GUIZHOU MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
GUIZHOU MEDICAL UNIV
Publication Date
2017-04-26

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Abstract

The invention discloses a screening method of anti myocardial ischemia active components of flower of Polygonum orientale, myocardial cells are used for selective combination with the active components of extract of the flower of Polygonum orientale, after other uncombined ingredients are washed away, cellular targets are inactivated, the combined active components are released from the myocardial cells, and then the UHPLC-Q-TOF / MS technology is used for analyzing and identifying. The method has the advantages that by adopting of the specific H9c2 myocardial cells, the active components of the extract of the flower of Polygonum orientale can be specifically and selectively combined with the specific H9c2 myocardial cells, the traditional Chinese medicine action multi-target screening of the active components can be met, by use of an UHPLC-Q-TOF / MS system, a reliable method of qualitative analysis of biological samples can be established, medicinal components in a myocardial cell extraction solution can be comprehensively reflected, and reference is provided for rapid determination of pharmacodynamic material basis of the flower of Polygonum orientale and innovative drug research.
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Description

technical field

[0001] The invention relates to the field of drug analysis, in particular to a screening method for anti-myocardial ischemia active ingredients of the sage flower. Background technique

[0002] Biological activity screening / chemical composition online analysis technology is a comprehensive technology formed by the intersection of life science, chromatography and mass spectrometry separation and identification technology. Active biological macromolecules, active cell membranes, and even active cells that play important physiological roles in life activities such as ion channels and nerve mediators are used as targets to establish a biological activity screening / chemical composition online analysis method based on the effective substances of traditional Chinese medicine. Cell extraction can use the selectivity of cells to screen the components that bind to cells. There is a significant correlation between the components that bind to cells and the effect compone...

Claims

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