Method for efficiently and quickly detecting cistanche seed germination rate

A technology of Cistanche deserticola and germination rate, which is applied in the field of efficient and rapid inspection of Cistanche deserticola seed germination rate, can solve the problems of low germination rate, long germination time, instability, etc., and achieve the effect of shortening germination time, increasing germination rate, and breaking dormancy

Active Publication Date: 2017-05-10
GANSU AGRI UNIV
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Problems solved by technology

Although these methods have broken the dormancy of Cistanche seeds to a certain extent, the germination rate is still low and unstable, and the germination time is too long. Even in the most ideal state, the highest germination rate of Cistanche tubulosa can reach 75%. The germination rate is the lowest, ranging from 16.7% to 52.5%, and the germination rate cannot be counted until 30d
The existing methods are not convenient for seed quality inspection, which seriously affects the market circulation of Cistanche seeds and increases the risk of farmers planting

Method used

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  • Method for efficiently and quickly detecting cistanche seed germination rate
  • Method for efficiently and quickly detecting cistanche seed germination rate

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Embodiment 1

[0028] Embodiment 1: a kind of method of high-efficiency rapid inspection Cistanche seed germination rate, concrete steps are as follows:

[0029] 1) Seed soaking in warm water: Soak desert cistanche seeds in warm water at 30°C for 4 hours, and dry the water on the surface of the seeds with filter paper;

[0030] 2) Fluoridinone treatment: Soak the Cistanche deserticola seeds obtained in step 1) at 25°C with 10 mg / L fluridone solution for 36 hours, then rinse with distilled water twice, and use filter paper to dry the water on the surface of the seeds for later use;

[0031] 3) Prepare medium: prepare a special medium for macronutrients, including KNO 3 , NH 4 NO 3 , MgSO 4 ·7H 2 O and KH 2 PO 4 , its concentration is respectively 950mg / L, 825mg / L, 185mg / L, 85mg / L, mix above-mentioned four kinds of components according to corresponding concentration, adjust pH value to be 5.8, add the agar that accounts for 0.8% of culture medium weight, stir evenly After high-pressure ...

Embodiment 2

[0036] Embodiment 2: Detect the impact of light conditions on the germination of desert cistanche seeds, the specific steps are as follows:

[0037] 1) Seed soaking in warm water: Soak desert cistanche seeds in warm water at 30°C for 4 hours, and dry the water on the surface of the seeds with filter paper;

[0038] 2) Fluoridinone treatment: Soak the Cistanche deserticola seeds obtained in step 1) at 25°C with 10 mg / L fluridone solution for 36 hours, then rinse with distilled water twice, and use filter paper to dry the water on the surface of the seeds for later use;

[0039] 3) Prepare medium: prepare 1 / 2MS special medium, use MS medium formula as the basis, halve the concentration of all inorganic salts in it, prepare 1 / 2MS basal medium, and then add 1 / 2MS basal culture Base weight 3% sucrose, adjust the pH value to 5.8, then add 0.8% agar accounting for 1 / 2MS basic medium weight, stir evenly and then autoclave to obtain 1 / 2MS special medium, the prepared medium Pack into ...

Embodiment 3

[0044] Embodiment 3: Detect the impact of different germination beds on the germination of desert cistanche seeds, the steps are as follows:

[0045] 1) Seed soaking in warm water: Soak desert cistanche seeds in warm water at 30°C for 4 hours, and dry the water on the surface of the seeds with filter paper;

[0046] 2) Fluoridinone treatment: Soak the Cistanche deserticola seeds obtained in step 1) at 25°C with 10 mg / L fluridone solution for 36 hours, then rinse with distilled water twice, and use filter paper to dry the water on the surface of the seeds for later use;

[0047] 3) preparation of germination bed: 1 / 2MS special culture medium is prepared with embodiment 2;

[0048] A. Paper bed: Use 3 layers of filter paper as the germination bed, fully wet with distilled water and set aside;

[0049] B. Absorbent cotton: Spread a thin layer (3mm thick) of absorbent cotton on the bottom of the culture dish, flatten it, and completely wet it with distilled water before use;

[...

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Abstract

The invention relates to a method for efficiently and quickly detecting cistanche seed germination rate. The method includes steps of seed soaking with warm water, treatment with fluridone, preparation of a culture medium, disinfection of seeds, placement of the seeds on a bed and germination. A 1/2 MS special culture medium is characterized in that an MS culture medium formulation is adopted as a base, and concentration of all inorganic salts therein is reduced by half; a macroelement special culture medium comprises KNO3, NH4NO3, MgSO47 H2O and KH2PO4 with concentration of 950mg/L, 825mg/L, 185mg/L and 85mg/L respectively, the above four components are mixed according to corresponding concentration, pH value is adjusted to 5.8, agar accounting for 0.8% of weight of the culture medium is added, and high-pressure damp-hot sterilization is performed after well mixing. The method is quick and efficient, and desert cistanche seed germination rate is up to 73% in 14d.

Description

technical field [0001] The invention relates to the technical field of agricultural economic crop breeding, in particular to a method for quickly and efficiently testing the germination rate of cistanche seeds. Background technique [0002] Cistanche deserticola has been used in China for 1,800 years as a Chinese herbal medicine. It is a rare and precious Chinese medicinal material in my country. At present, there are three kinds of crude drugs of Cistanche commonly used in the crude drug market, namely Desert Cistanche, Cistanche tuberosa and Salt Cistanche. Cistanche is a perennial specific root parasitic plant. The embryo of mature Cistanche seeds is a spherical proembryo with simple development and no differentiation of radicle, germ and cotyledons. Germination under natural conditions requires a certain period of morphological maturation and physiological Dormancy can only be lifted after the post-ripening period. Due to the complex dormancy characteristics and specia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01C1/02
CPCA01C1/02A01C1/025A01H4/001A01H4/008
Inventor 郭晔红杜弢张琴玲
Owner GANSU AGRI UNIV
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