Thick broad-bean sauce chilli billet low-salt fermentation method based on microbial augmentation and original pool pouring
A pool pouring and microbial technology, which is applied in the field of low-salt fermentation of watercress and pepper blanks, to achieve the effect of avoiding fermentation instability and reducing salt concentration
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Embodiment 1
[0022] A low-salt fermentation method for watercress and chilli slabs based on microbial intensification and pouring in the original pond. The steps are:
[0023] (1) Pretreatment of pepper blanks
[0024] Take the freshly harvested red peppers of the season, remove the peppers, clean, drain, crush, add 13-18% w / w edible salt and mix evenly into the storage tank with false bottom for salting for 3-10 days;
[0025] (2) Preparation of composite microbial fermentation broth
[0026] Xanthomonas sp. CICC 10257 was cultured in Xanthomonas culture medium at 30℃ for 48-72h, so that the total number of viable bacteria reached 10 9 Above CFU / g;
[0027] Saccharomyces cerevisiae CICC 31016 was cultured in Saccharomyces cerevisiae medium at 30℃ for 16-24h, so that the total number of viable bacteria reached 10 8 Above CFU / g;
[0028] Bacillus subtilis (Bacillus subtilis CICC 20520) and Lactobacillus plantarum (Lactobacillusplantarum BNCC194165) were cultured in the combined medium at 37℃ for 48-72...
Embodiment 2
[0037] Attachment 2, Testing methods for volatile components such as alcohols, aldehydes, acids, esters, etc.
[0038] Solid-phase microextraction device, Supelco, USA; QP2010plus gas chromatography-mass spectrometer, Shimadzu Corporation, Japan; Constant temperature circulation tank, Shanghai Yiheng Scientific Instrument Co., Ltd.; 90mm mortar, Kaiping Shengxing Chemical Porcelain Factory, Tangshan City.
[0039] 1 sample pretreatment
[0040] Take about 20g of watercress with a homogeneous sample, grind it in a mortar to a uniform paste, seal the bag and store it at -20°C. All samples should be tested within 3 days after retrieval.
[0041] 2HS-SPME extraction method
[0042] Weigh 5g of the ground sample, put it into a 15mL sealed headspace bottle, and equilibrate it in a 55°C water bath for 20 minutes. Then insert the aged 75μm CAR / PDMS extraction head into the headspace bottle for 30min adsorption, and then insert the GC-MS injection port for 5min analysis.
[0043] 3GC-MS analysi...
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