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Medium for ningnanmycin producing strain and preparation method of medium

A technology of Ningnanmycin and culture medium, which is applied in the field of culture medium and preparation of Ningnanmycin-producing bacteria, can solve the problems of thinning of bacterial lawn, complicated preparation process, poor growth, etc., and achieve plump and thick spores, improve Cultivate quality, low-cost effects

Inactive Publication Date: 2017-05-10
陕西麦可罗生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many kinds of ingredients in the original medium for producing bacteria, and the preparation process is complicated
The lawn was plump and thick after being inoculated on the original medium, but the lawn became thinner after subculture, and even did not produce spores after several generations, which affected the quality of the seeds and failed to meet the production requirements
In order to solve the current problem of poor growth, it is of great significance to seek a simple, more suitable medium for the growth, stability and sporulation of Ningnanmycin-producing bacteria.
However, in the prior art, there are few studies on the culture medium for Ningnanmycin producing bacteria

Method used

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  • Medium for ningnanmycin producing strain and preparation method of medium
  • Medium for ningnanmycin producing strain and preparation method of medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Weigh 4g of corn flour, 6g of maltose, 3g of yeast extract, and 16g of agar, put them all into 1L of distilled water, heat to dissolve, add water to volume, adjust the pH value to 7.5, autoclave at 121°C for 20min, and cool to obtain Ningnanmycin Sporulation medium for producing bacteria. Ningnanmycin-producing bacteria were inoculated and cultured at 28°C for 7 days. With the original medium (1L contains 5g of soluble starch, 5g of glucose, 2.5g of corn flour, 2g of hot-pressed peanut cake powder, 0.5g of beer yeast powder, (NH 4 ) 2 SO 4 2.5g, MgSO 4 ·7H 2 O25mg, K 2 HPO 4 0.2g, CaCO 3 3g, agar 20g), the bacterial lawn is more abundant, and the spores are produced faster and more. After several passages, no spore production occurred.

[0016] The comparison of table 1 embodiment 1 with the original culture medium

[0017]

Embodiment 2

[0019] Weigh 6g of corn flour, 2g of maltose, 6g of yeast extract, and 20g of agar, put them all into 1L of distilled water, heat to dissolve, add water to volume, adjust the pH value to 7.0, autoclave at 121°C for 20min, and cool down to obtain Ningnanmycin Sporulation medium for producing bacteria. Ningnanmycin-producing bacteria were inoculated and cultured at 28°C for 7 days. With the original medium (1L contains 5g of soluble starch, 5g of glucose, 2.5g of corn flour, 2g of hot-pressed peanut cake powder, 0.5g of beer yeast powder, (NH 4 ) 2 SO 4 2.5g, MgSO 4 ·7H 2 O25mg, K 2 HPO 4 0.2g, CaCO 3 3g, agar 20g), the bacterial lawn is more abundant, and the spores are produced faster and more.

[0020] The comparison of the culture medium of table 2 embodiment 2 and former culture medium

[0021]

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Abstract

The invention relates to a medium for a ningnanmycin producing strain and a preparation method of the medium. The medium comprises the following raw materials, by weight part, 0.4-0.8% of corn flour, 0.2-0.8% of maltose, 0.3-0.9% of extract yeast, and 1.6-2.0% of agar, with the balance being distilled water. Carbon sources and nitrogen sources are comprehensively used in the medium to increase the sporulation quantity of ningnanmycin.

Description

technical field [0001] The invention belongs to the field of microbial strain cultivation, and in particular relates to a culture medium for Ningnanmycin-producing bacteria and a preparation method thereof. Background technique [0002] Ningnanmycin is a new type of biological pesticide with broad spectrum, low toxicity, low residue, no "three causes" and accumulation effect. It was discovered and successfully developed by Chengdu Institute of Biology, Chinese Academy of Sciences and has independent intellectual property rights. Cytosine nucleoside peptide-type new antibiotics is a key scientific and technological project of the country's "Seventh Five-Year Plan", "Eighth Five-Year Plan" and "Ninth Five-Year Plan". Ningnanmycin has the functions of sterilizing, anti-virus, regulating and promoting growth, and can effectively prevent and control viral diseases in various crops such as tobacco, tomato, pepper, melon, rice, wheat, vegetables, fruit trees, beans, etc. Powdery m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P21/02
CPCC12N1/14C12P21/02
Inventor 杨玉旺李蒲民
Owner 陕西麦可罗生物科技有限公司