Litchi leaf specific expression gene LcGRX promoter and application thereof

A technology for expressing genes and promoters, applied in the field of promoters, can solve problems such as affecting the normal growth and development of plants, plant death, and breaking the metabolic balance of plants
CN106636091AInactive Publication Date: 2017-05-10ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
Publication Date
2017-05-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a litchi leaf specific expression gene LcGRX promoter and application thereof. The invention provides (1) a method for obtaining a primer sequence of the litchi leaf specific expression gene LcGRX promoter, (2) a construction method of a recombinant expression vector which takes pCAMBIA1304 as a basic expression vector and contains the LcGRX promoter and an LcTLP gene segment, and (3) a method for verifying the function of the promoter by using biolistic transformation and GUS transient gene expression. An experiment shows that the litchi leaf specific expression promoter is cloned from litchi and is verified to be a promoter for tissue specific expression, so that a basis is supplied to further transgenic breeding of litchi.
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Description

technical field

[0001] The invention relates to the field of promoters, in particular to a promoter of litchi leaf-specific expression gene LcGRX and its application. Background technique

[0002] Litchi is an important fruit tree in South China and occupies an important position in the agricultural economy. However, the pre-harvest fruit is vulnerable to the damage of pedicle borers and other hazards, which leads to serious fruit drop, thereby reducing the yield of litchi; after harvest, the litchi fruit is not resistant to storage, the skin is browned, the disease is serious, and the shelf life of litchi is shortened due to water loss and cracking. , which reduces the commodity value of litchi and limits the development of litchi industry. Breeding new varieties with multi-resistance, high-quality and storability is the fundamental way to solve these problems. However, due to the narrow genetic base of litchi and the lack of resistance resources, the progress of resistan...

Claims

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