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A method for patterning platelets on the surface of materials using polyphenolic compounds

A technology of polyphenolic compounds and polyphenolic compounds, which is applied in the direction of analysis materials, test sample preparation, sampling, etc., can solve the problem of platelet adhesion that has not been seen yet, and achieve the effect of low cost, simple and fast operation

Active Publication Date: 2019-06-25
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is no similar report on the regulation of platelet adhesion behavior by transferring polyphenolic materials to the surface of target materials

Method used

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  • A method for patterning platelets on the surface of materials using polyphenolic compounds
  • A method for patterning platelets on the surface of materials using polyphenolic compounds
  • A method for patterning platelets on the surface of materials using polyphenolic compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1) With 50% aqueous ethanol as a solvent, configure a concentration of 3.2 mg / mL of tannic acid solution and 0.8 mg / mL ferric chloride solution respectively, then mix the two solutions in equal volumes to obtain tannic acid- Iron ion solution ( figure 1 a);

[0032] 2) Add the tannic acid-iron ion solution dropwise to the surface of the silicone rubber stamp ( figure 2 ), using a spin coater to modify the surface of the stamp ( figure 1a), the parameters of the spin coater are controlled at 2000rpm / min, and the total drip volume of the tannic acid-iron ion solution is 1mL / mm 2 , the modification time is 3min;

[0033] 3) The surface of the stamp modified with tannic acid material is attached to the surface of the target material silicon wafer, so that the tannic acid material is transferred to the surface of the silicon wafer, and the stamp is removed after 10 minutes to obtain a silicon wafer with a micro-pattern of tannic acid surface;

[0034] 4) A silicon wafe...

Embodiment 2

[0038] 1) With 50% aqueous ethanol as a solvent, configure a concentration of 0.8 mg / mL epicatechin gallate solution and a 0.8 mg / mL ferric chloride solution respectively, then mix the two solutions in equal volumes to obtain Epicatechin gallate-iron ion solution;

[0039] 2) Add epicatechin gallate-iron ion solution dropwise to the surface of the silicone rubber stamp with a micro-pattern (strip interval of 20 microns), and use a spin coater to modify the surface of the stamp, and the parameters of the spin coater are controlled At 2000rpm / min, the total drip volume of epicatechin gallate-iron ion solution is 1mL / mm 2 , the modification time is 3min;

[0040] 3) The surface of the stamp modified with the epicatechin gallate material is attached to the surface of the silicon wafer of the target material, so that the epicatechin gallate material is transferred to the surface of the silicon wafer, and the stamp is removed after 10 minutes to obtain a Silicon wafer surface micr...

Embodiment 3

[0043] 1) Using 50% aqueous ethanol as a solvent, prepare respectively an epigallocatechin gallate solution with a concentration of 3.2 mg / mL and a ferric chloride solution with a concentration of 0.8 mg / mL, then mix the two solutions in equal volumes, Obtaining epigallocatechin gallate-iron ion solution;

[0044] 2) Add epigallocatechin gallate-iron ion solution dropwise to the surface of the silicone rubber stamp with a micro-pattern (strip interval of 20 microns), and use a spin coater to modify the surface of the stamp. The parameters of the spin coater Controlled at 2000rpm / min, the total drip volume of epigallocatechin gallate-iron ion solution is 1mL / mm 2 , the modification time is 3min;

[0045] 3) The surface of the stamp modified with the epigallocatechin gallate material is attached to the surface of the silicon wafer of the target material, so that the epigallocatechin gallate material is transferred to the surface of the silicon wafer, and the stamp is removed af...

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Abstract

The invention provides a method for realizing platelet patterning by polyphenols on material surface. According to the method, a polyphenol and metal ion mixed solution is prepared under chelation between the polyphenols and metal ions and is added dropwise to a seal surface with a micro-pattern, a patterned seal made of the polyphenol material is obtained and finally used for performing micro-contact printing on a target material, and the patterned polyphenol material surface is obtained. After the patterned polyphenol material surface makes contact with platelets, the platelets selectively adhere to areas not covered with the polyphenol material, so that patterning regulation of the platelets is realized. Hydrophilic polymers or bioactive proteins need not to be introduced, and the method has the characteristics of being low in cost and simple and convenient to operate. The prepared platelet patterned surface is expected to be applied to fields such as platelet adhesion detection, antiplatelet drug therapy and evaluation and the like.

Description

technical field [0001] The invention relates to biomaterial surface modification technology and surface micro-pattern preparation technology, in particular to the technology of regulating platelet adhesion behavior on the material surface. Background technique [0002] Platelets are biologically active small pieces of cytoplasm produced by megakaryocytes in the bone marrow. Under physiological conditions, platelets perform functions such as adhesion and aggregation to ensure the smooth progress of normal hemostasis. At the same time, platelet adhesion is also related to pathological thrombosis, tumor metastasis, inflammation, etc. and immune responses. Therefore, it is often necessary to detect the adhesion properties of normal or abnormal platelets in clinical practice. However, in vitro studies, the adhesion behavior of platelets is affected by many factors, such as the limitation of spatial geometric configuration, the interaction between platelets and the substrate role...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N11/00
CPCG01N1/2813G01N11/00G01N2001/282
Inventor 贾凌云杨立为韩璐璐任军
Owner DALIAN UNIV OF TECH
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