Method for identifying acacia honey producing place by non-target metabonomics technology

A kind of technology of acacia honey and origin, applied in the direction of scientific instruments, measuring devices, instruments, etc., can solve unscientific problems, achieve the effect of ensuring health and improving the quality of honey

Active Publication Date: 2017-05-10
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is very limited and unscientific to find a reliable marker to identify its botanical and geographical origin based on a class of compounds based on prior subjective assumptions

Method used

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  • Method for identifying acacia honey producing place by non-target metabonomics technology
  • Method for identifying acacia honey producing place by non-target metabonomics technology
  • Method for identifying acacia honey producing place by non-target metabonomics technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] A method for screening characteristic markers used to identify the origin of acacia honey, wherein the origin refers to Shaanxi and Liaoning, and the screening method comprises the following steps:

[0085](1) A total of 60 honey samples were collected from Liaoning and Shaanxi, the main producing areas of Yanghuai honey in my country. Among them, there are 27 samples from Liaoning Province and 33 samples from Shaanxi Province. The samples were stored in a well-ventilated sample storage room at 20°C and protected from light. In order to improve the representativeness of honey samples, 3-6 honey samples of the same honey source plants in the same place of origin are randomly and uniformly mixed into one sample, and finally there are 5 honey samples with the same plant source in each place of origin, and 5 honey samples from each place of origin The samples are divided into one group, two groups in total;

[0086] (2) Weigh 1.0±0.1g of each honey sample and place them i...

Embodiment 2

[0099] A method for screening characteristic markers used to identify the origin of acacia honey, wherein the origin refers to Shaanxi and Liaoning, the screening method is the same as in Example 1, the only difference is that the sample processing method is different and the analysis conditions are different, The conditions for this example are as follows:

[0100] (1) Weigh 2.0±0.1g of each portion of honey and place it in a 10mL stoppered centrifuge tube, add 8mL of pure water, vortex for 8 minutes to mix, centrifuge at 8000rpm for 10 minutes to precipitate particulate impurities, and take the supernatant to obtain the product;

[0101] (2) High performance liquid chromatography:

[0102] Mobile phase: 0.1% formic acid water (A) and 0.1% formic acid acetonitrile solution (B);

[0103] Elution conditions: 0–2.0min, 5%B; 2.0–7.0min, 5%–30%B; 7.0–13.0min, 30%–95%B; 13.0–18.0min, 95%B; 18.0–18.1min ,95%-5%B; 18.1-20.0min, 5%B.;

[0104] Chromatographic column: Thermo Scienti...

Embodiment 3

[0110] A method for screening characteristic markers used to identify the origin of acacia honey, wherein the origin refers to Shaanxi and Liaoning, the screening method is the same as in Example 1, the only difference is that the sample processing method is different and the analysis conditions are different, The conditions for this example are as follows:

[0111] (1) Weigh 3.0±0.1g of each portion of honey and place it in a 10mL stoppered centrifuge tube, add 10mL of pure water, vortex for 10 minutes to mix, centrifuge at 9000rpm for 8 minutes to precipitate particulate impurities, and take the supernatant to obtain the product;

[0112] (2) High performance liquid chromatography:

[0113] Mobile phase: 0.08% formic acid water (A) and 0.08% formic acid acetonitrile solution (B);

[0114] Elution conditions: 0–2.0min, 5%B; 2.0–7.0min, 5%–30%B; 7.0–13.0min, 30%–95%B; 13.0–18.0min, 95%B; 18.0–18.1min ,95%-5%B; 18.1-20.0min, 5%B.;

[0115] Chromatographic column: Thermo Scie...

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Abstract

The invention relates to a method for screening a feature marker for identifying a honey producing place. According to the method, a simple sample pre-treatment process is performed; then, a great amount of high-resolution mass spectrum data is obtained by an ultra-high performance liquid chromatography-quadrupole/electrostatic field orbital hydrazine high-resolution mass spectrometry; deviation and variation interference in experiment data are eliminated through background removal, chromatographic peak extraction, normalization, data conversion and data zooming; then, through main ingredient analysis, t-inspection, Volcano plots and variable importance projection drawing, the real feature markers capable of distinguishing the same honey source honey from different producing places can be discovered and verified. After the feature marker is used for identifying the honey producing place, identification results are objective and precise; the method can be used for the variety source tracing identification of honey of the same honey source from different producing places, and can also be used for the variety source tracing and producing place tracing identification of other farm products.

Description

technical field [0001] The invention relates to the technical field of honey origin identification, in particular to a method for screening characteristic markers for identifying honey origins, and also to a method for using the characteristic markers to identify honey origins. In particular, it relates to a method for screening characteristic markers for identifying the origin of acacia honey, and a method for identifying the origin of acacia honey. Background technique [0002] my country is a big beekeeping country in the world, with an annual output of more than 450,000 tons of honey. It is a big country in honey production, consumption and export. Studies have shown that honey contains nearly a hundred kinds of endogenous components, glucose and fructose account for more than 70% of the total component content, followed by water, sucrose, minerals, vitamins, proteins, amino acids, etc. Consumer interest in honey has been increasing in recent years mainly because of its...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88G01N2030/062G01N2030/8809
Inventor 周金慧李熠问亚琴张文文陈兰珍张金振金玥赵文
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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