A method for characterizing microbe phagocytosis by pomfret macrophages

A technology of macrophages and microorganisms, applied in the field of cell biology, can solve the problems of isolation, purification, culture and characterization of macrophages in the kidney of pompano pomfret head that have not been seen

Inactive Publication Date: 2018-11-02
SHENZHEN INST OF GUANGDONG OCEAN UNIV +2
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  • Abstract
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Problems solved by technology

[0008] There are few reports on the immune function of S. ovata. The head kidney is an important immune organ of fish. There are no reports on the isolation, purification, culture and characterization of macrophages from the head kidney of S. ovata.

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  • A method for characterizing microbe phagocytosis by pomfret macrophages
  • A method for characterizing microbe phagocytosis by pomfret macrophages
  • A method for characterizing microbe phagocytosis by pomfret macrophages

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Embodiment Construction

[0070] In order to better explain the technical solutions of the present invention, various embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings. The following examples are used to further illustrate the present invention, but should not be construed as fixing or limiting the present invention. Unless otherwise specified, the technical features used in the embodiments may be replaced with other technical features known in the art that have equivalent or similar functions or effects without departing from the inventive concept.

[0071] 1. Experimental materials

[0072] 1.1 Experimental fish

[0073] Oval pomfret, commonly known as golden pomfret. The body is pomfret-shaped, high and flat on the sides; the body length is 1.7-1.9 times the body height and 3.8 times the head length. The tail peduncle is short and thin, and the side is flat. Head small, taller than long. The occipital crest is prominent. The he...

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Abstract

The invention discloses a method for characterizing a microorganism phagocytosis function of macrophages of trachinotus ovatus. The trachinotus ovatus is dissected and separated, and the macrophages of the head kidney of the trachinotus ovatus are obtained and are extracted with a Percoll solution density gradient centrifugation method. The number of survived cells cultured in vitro is calculated with a trypan blue staining method, and the survival condition of the cells in one week is detected. The phagocytosis of the macrophages of the head kidney of the trachinotus ovatus on Nocardia seriolea, saccharomycetes and the like is determined through a phagocytosis experiment, so that the phagocytosis function of the macrophages is observed. An experimental result shows that the macrophages of the trachinotus ovatus can be extracted very well, and the survival condition and the phagocytosis condition of the macrophages of the trachinotus ovatus can be observed very well. A foundation is laid for further research of physiology, pathology and toxicology of the macrophages of the trachinotus ovatus.

Description

technical field [0001] The invention belongs to the field of cell biology and relates to a method for characterizing the interaction between immune cells and microorganisms, in particular to a method for characterizing the phagocytosis of microorganisms by pomfret macrophages. Background technique [0002] Oval pomfret (Trachinotus ovatus) (Linnaeus), commonly known as golden pomfret, yellow wax pomfret, belongs to Osteichthyes, Vertebrate Phylum, Osteichthyes, Perciformes, Trevalidae, Pomfrets genus. Oval pomfret has a flat side, oval shape, large body, fast growth, and strong disease resistance. In natural waters, pompano pomfret prefers pomfret pomfret to forage and migrate in clusters, vying for food fiercely, mainly feeding on small molluscs and small snails. The large individual weighs 5-10kg, and the meat is tender and delicious. It is a valuable edible fish. [0003] Pomfret ovata is a seawater fish with strong disease resistance, and there were few reports on its ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50G01N15/10G01N1/28
CPCG01N1/2813G01N15/10G01N33/5055G01N2015/1006
Inventor 夏立群鲁义善简纪常蔡佳
Owner SHENZHEN INST OF GUANGDONG OCEAN UNIV
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