A method for characterizing microbe phagocytosis by pomfret macrophages

A technology of macrophages and microorganisms, applied in the field of cell biology, can solve the problems of isolation, purification, culture and characterization of macrophages in the kidney of pompano pomfret head that have not been seen
CN106645669BInactive Publication Date: 2018-11-02SHENZHEN INST OF GUANGDONG OCEAN UNIV +2
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHENZHEN INST OF GUANGDONG OCEAN UNIV
Publication Date
2018-11-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for characterizing a microorganism phagocytosis function of macrophages of trachinotus ovatus. The trachinotus ovatus is dissected and separated, and the macrophages of the head kidney of the trachinotus ovatus are obtained and are extracted with a Percoll solution density gradient centrifugation method. The number of survived cells cultured in vitro is calculated with a trypan blue staining method, and the survival condition of the cells in one week is detected. The phagocytosis of the macrophages of the head kidney of the trachinotus ovatus on Nocardia seriolea, saccharomycetes and the like is determined through a phagocytosis experiment, so that the phagocytosis function of the macrophages is observed. An experimental result shows that the macrophages of the trachinotus ovatus can be extracted very well, and the survival condition and the phagocytosis condition of the macrophages of the trachinotus ovatus can be observed very well. A foundation is laid for further research of physiology, pathology and toxicology of the macrophages of the trachinotus ovatus.
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Description

technical field

[0001] The invention belongs to the field of cell biology and relates to a method for characterizing the interaction between immune cells and microorganisms, in particular to a method for characterizing the phagocytosis of microorganisms by pomfret macrophages. Background technique

[0002] Oval pomfret (Trachinotus ovatus) (Linnaeus), commonly known as golden pomfret, yellow wax pomfret, belongs to Osteichthyes, Vertebrate Phylum, Osteichthyes, Perciformes, Trevalidae, Pomfrets genus. Oval pomfret has a flat side, oval shape, large body, fast growth, and strong disease resistance. In natural waters, pompano pomfret prefers pomfret pomfret to forage and migrate in clusters, vying for food fiercely, mainly feeding on small molluscs and small snails. The large individual weighs 5-10kg, and the meat is tender and delicious. It is a valuable edible fish.

[0003] Pomfret ovata is a seawater fish with strong disease resistance, and there were few reports on its ...

Claims

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