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Quantitative detection calculation method based on fluorescent immuno-chromatographic technology

A fluorescence immunochromatography and quantitative detection technology, which is applied in the direction of fluorescence/phosphorescence, material analysis through optical means, and measuring devices, etc., can solve the problems of reduced reliability of detection values, large errors of fluorescence immunochromatography, and reduction of fluorescence immune layer Analyze technical application issues and other issues to achieve the effects of low power consumption, reduced stray light interference, and high stability

Inactive Publication Date: 2017-05-10
BIOHIT BIOTECH HEFEI
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Problems solved by technology

In the algorithm processing after the existing fluorescence immunochromatography technology, there is generally no targeted filtering operation for noise interference, which leads to large errors in the detection of fluorescence immunochromatography and reduces the reliability of the detection value, thereby reducing the fluorescence immune layer. Application of Analysis Technology

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  • Quantitative detection calculation method based on fluorescent immuno-chromatographic technology
  • Quantitative detection calculation method based on fluorescent immuno-chromatographic technology
  • Quantitative detection calculation method based on fluorescent immuno-chromatographic technology

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Embodiment Construction

[0044]In order to better understand the present invention, the present invention will be further described below in conjunction with the examples and accompanying drawings, and the following examples are only to illustrate the present invention rather than limit it.

[0045] A kind of quantitative detection calculation method based on fluorescence immunochromatography technology of the present invention, such as figure 1 shown, including the following steps:

[0046] 1. Reagent strip loading

[0047] Drop at least 40 microliters of the sample solution to be tested on the fluorescent immunoassay strip, let it stand for 15 minutes, and then insert it into the instrument to start detection.

[0048] 2. The hardware processing flow in the instrument is as follows:

[0049] (1) When the LED light source illuminates the test paper window on the reagent strip, the excitation wavelength of the fluorescent substance on the reagent strip is mainly concentrated at about 365nm, and the ...

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Abstract

The invention relates to the field of immuno-chromatographic detection, in particular to a quantitative detection calculation method based on fluorescent immuno-chromatographic technology. The method includes the steps: inserting a fluorescent immuno-chromatographic test strip dripping sample solution to be measured into a fluorescent immunity analyzer; irradiating by the aid of LED (light-emitting diode) light sources; performing light filtering treatment by the aid of a light filter; detecting by the aid of a photoelectric detector; treating electric signals; performing AD conversion treatment; performing filtering algorithm treatment; performing baseline fitting; performing peak searching treatment; calculating T / C area ratio; calculating concentration; judging by the aid of calculated concentration results. The invention provides a perfect, rapid and quantitative detection calculation method based on the fluorescent immuno-chromatographic technologies. Various noise and signal interference can be filtered when concentration of objects to be measured is detected and calculated, and accurate detection results are improved. The method has the advantages of high accuracy and stability and low power consumption, and rapid and quantitative detection requirements of users are effectively met.

Description

technical field [0001] The invention relates to the field of immunochromatography detection, in particular to a quantitative detection and calculation method based on fluorescence immunochromatography technology. Background technique [0002] As the pace of social operation is getting faster and faster, whether it is the on-site detection of disease epidemics, the on-site rapid detection of toxic and harmful substances in food, the clinical detection of emergency rescue patients in emergency departments and ICUs, and the emergency detection and rescue of disaster medicine , and in the detection of alcohol and drug concentration at the scene of the accident; there is an urgent need for a rapid on-site detection technology that is fast to detect, easy to carry, and easy to operate. However, due to the ubiquitous defects such as narrow measurable range and instability, the traditional rapid detection technology cannot well meet the requirements of quantitative rapid detection...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N21/64
CPCG01N33/558G01N21/6428
Inventor 应玉龙洪津夏果陈迪虎裘祯炜张继尧
Owner BIOHIT BIOTECH HEFEI
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