Concentration gradient generating device and method for generating concentration gradient in one step

Abstract

The invention discloses a concentration gradient generating device and a method for generating a concentration gradient in one step. The concentration gradient generating device comprises first and second micro-channel networks which are arranged along a vertical direction, wherein the first and second micro-channel networks respectively comprise a plurality of first and second concentration generating channels; one end of each first concentration generating channel is communicated with a first sample inlet of the first micro-channel network; one end of each second concentration generating channel is communicated with a second sample inlet of the second micro-channel network; first and second fluids are respectively injected into the first sample inlet and the second sample inlet; the other end of each first concentration generating channel is communicated with the other end of the corresponding second concentration generating channel and also is connected with a connecting channel, so that the first and second fluids can be converged in the connecting channel; and each connecting channel is also connected with a mixing channel. The concentration gradient generating device disclosed by the invention has the advantages of simple structure, high stability, easiness in manufacturing, and the like. The samples in various target concentrations can be formed in one step.

Description

Technical field [0001] This application relates to a concentration gradient generating device, in particular to a device and method that can generate a concentration gradient in one step, belonging to the technical fields of biology and analytical chemistry. Background technique [0002] The preparation of concentration gradient solutions in biochemical experiments is one of the most basic operations. In cell experiments and drug screening experiments, the behavior and toxicity of different concentrations of biomolecules and drugs to cells are quite different. Therefore, when investigating the effects of different concentrations of biomolecules and drug dosages on cell behavior and toxicity, it is necessary to prepare a set of target solutions with a gradient distribution. The traditional concentration gradient preparation method requires accurate calculation, weighing, and multiple solution mixing. It requires a lot of chemical containers, cumbersome operation, and cumbersome op...

AI Technical Summary

Problems solved by technology

However, this kind of concentration gradient generating device has a complex microchannel network structure, which requires a long straight channel or a complicated micromixer to achieve each separation and mixing process of the fluid, and its structure complexity and complexity increase with the increase of the target concentration. The number of microchannels has also increased, resulting in a complex structure, inconvenient manufacture, and large redundancy of the concentration gradient generation device; the concentration gradient generation device based on molecular diffusion is simple in structure, easy to operate, and easy to manufacture and process. The principle of diffusion, the concentration gradient generated by this chip will change with time, it is difficult to obtain a stable concentration gradient, and the flow rate, molecular size, liquid viscosity and diffusion distance all have a large influence on the generation of concentration gradient influences

Images