Diterpenoid alkaloid compounds in Aconitum naviculare as well as preparation method and application of diterpenoid alkaloid compounds

A technology of diterpene alkaloids, compounds, applied in the field of phytochemistry

Inactive Publication Date: 2017-05-17
KUNMING UNIV
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aconitum aconitum is widely used. At present, there are few reference data for systematic research on the chemical components and pharmacological effects of this Tibetan medicine. Therefore, the diterpene alkaloid components with anti-tumor activity in Aconitum aconitum are extracted Separation research is necessary, and the exploration of the antitumor activity of Aconitum aconitum has great academic value

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Diterpenoid alkaloid compounds in Aconitum naviculare as well as preparation method and application of diterpenoid alkaloid compounds
  • Diterpenoid alkaloid compounds in Aconitum naviculare as well as preparation method and application of diterpenoid alkaloid compounds
  • Diterpenoid alkaloid compounds in Aconitum naviculare as well as preparation method and application of diterpenoid alkaloid compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] Take 10 kg of the aboveground parts of Aconitum chinensis, dry, pulverize, and cold soak in industrial methanol for 3 times and industrial acetone for 1 time, each time for 72 hours. The solvent is recovered to obtain the extract, then adjusted with acid water to pH=2-3, followed by extraction with petroleum ether, ethyl acetate and n-butanol. The acid water part was adjusted to PH=10, and then extracted with ethyl acetate and n-butanol again to obtain 18 g of total alkaloids in the n-butanol part. Total alkaloids were separated and analyzed by gel Sephadex LH-20 chromatography and reversed-phase RP-18 column chromatography.

[0019] The diterpene alkaloid compounds in the obtained Aconitia chinensis were analyzed, and Aconitin A(1) passed through 13 CNMR (Table 2) and ESIMS (m / z[M+H] + =623), it can be determined that its molecular formula is C 40 H 50 N 2 O 4 The degree of unsaturation was 15, and its planar structure was determined by 2D NMR. The relative conf...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to diterpenoid alkaloid compounds in Aconitum naviculare as well as a preparation method and an application of the diterpenoid alkaloid compounds. According to the method, crude Aconitum naviculare medicine is taken as a raw material and is extracted with a solvent, an extract is subjected to acid dissolution and alkali precipitation, a product is processed with gel Sephadex LH-20 chromatography and reversed-phase RP-18 column chromatography separation, three diterpenoid alkaloid compounds including C40H50N2O4 (1), C47H56N2O5 (2) and C29H37NO3 (3) are obtained after analysis with thin-layer chromatography, in-vitro cytotoxic activity of the three compounds is determined, and test results prove that C47H56N2O5 (2) has anti-tumor activity in human promyelocytic leukemia cells, human hepatoma cells, human lung cancer cells, human breast cancer cells and human colon cancer cells, and can be used for preparing anti-tumor drugs.

Description

technical field [0001] The invention relates to the field of phytochemistry, in particular to the extraction and separation of diterpene alkaloid compounds from the medicinal materials of Aconitum chinensis. Background technique [0002] Aconitum naviculare.Stapf, known as Bangga in Tibetan medicine, was first recorded in the classics of Tibetan medicine "Yuewang Medicine Clinic". It belongs to the genus Aconitum. of the family Ranunculaceae. Aconitum chinensis is bitter in taste, cold, and has a small poison. The application of this plant in Tibetan medicine is mostly compound preparations, such as the eleven flavors of golden pills, twelve flavors of Yishousan, and Thirteen flavors of Bangga powder. The 41 Tibetan medicinal preparations in the 2010 edition of the Chinese Pharmacopoeia all contain Aconitum chinensis, accounting for about 20% of the Tibetan medicinal preparations, which shows the extensiveness of its application. Modern pharmacological studies have shown t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/08C07D221/22A61P35/00
CPCC07D487/08C07D221/22
Inventor 何江波吕小满句红萍陶剑余珊王波张雪梅朱华结
Owner KUNMING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap