KIT gene mutation detection kit

Abstract

The invention provides a KIT gene mutation detection kit. The KIT gene mutation detection kit comprises: (1) specific primers used for amplifying five exons, namely an Exon9, an Exon11, an Exon13, an Exon 14 and an Exon 17, of a KIT gene in a sample, wherein the sequences of the primers are respectively shown as SEQ ID Nos:1-10; (2) a sequencing primer used for sequencing a PCR, and sequencing and analyzing the five exons of the KIT gene, wherein the sequencing primer is shown as SEQ ID Nos:11-15; (3) five positive quality control tubes and one test tube, wherein KIT wild type plasmids and mutant type plasmids are mixed in the five positive quality control tubes at the mass ratio of 1:1, and the test tube is filled with a PCR reaction premixing solution. The PCR amplification conditions of the different exons are consistent, the target gene can be detected when the concentration of the sample is relatively low, and no nonspecific amplification is found in the result.

Description

technical field [0001] The invention relates to a kit, in particular to a kit for detecting the mutation of a tumor-related gene KIT gene. Background technique [0002] Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor of the digestive tract. Most GISTs express the Kit protein (CD117) encoded by the c-kit gene. The c-kit gene is located on human chromosome 4q12-13. It is a proto-oncogene and its product is type III tyrosine kinase. At the molecular level, most GISTs have mutations in the c-kit gene, which leads to the activation of the kit protein without the participation of the ligand SCF to stimulate the continued proliferation of tumor cells and the loss of control of anti-apoptotic signals. [0003] Glivec (Glivec) is a tyrosine kinase inhibitor approved by the FDA for the treatment of c-kit positive, unresectable and / or metastatic malignant GIST, and its clinical efficacy is exciting. Its mechanism of action is that the drug binds to the ATP ...

AI Technical Summary

Problems solved by technology

Among them, the RT-PCR technology uses the allele-specific amplification method to distinguish the gene mutation of the sample. This method is low in cost, but the detection rate is high, and it is prone to false negative results; ARMS technology is currently the most widely used technology in China. , but only known sites can be detected, and the typing can only be achieved by dividing the sample into multiple tubes for experiments, which requires a high sample volume; while Sanger sequencing is a classic method of DNA sequence analysis, the most direct and detectable A method for known and unknown mutations

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