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Immunogen for obtaining nrf1d protein antibody, nrf1d protein antibody and elisa detection kit

A detection kit, immunogen technology, applied in the field of antibodies, to achieve the effect of high specificity and sensitivity

Active Publication Date: 2020-11-24
CHONGQING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the biggest problem at present is that there is no suitable reagent to detect the expression of Nrf1D protein

Method used

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  • Immunogen for obtaining nrf1d protein antibody, nrf1d protein antibody and elisa detection kit
  • Immunogen for obtaining nrf1d protein antibody, nrf1d protein antibody and elisa detection kit
  • Immunogen for obtaining nrf1d protein antibody, nrf1d protein antibody and elisa detection kit

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Embodiment Construction

[0021] The present invention will be further described in conjunction with the specific embodiments. In the case of the present embodiment of the present invention is a technical premise embodiment, details are now given of specific embodiments and processes of the present invention will be described operation of creative, although the scope of the present invention is not limited to the following examples.

[0022] First, experimental materials

[0023] 1, the main agent:

[0024] Sulfo-SMCC, KLH, SulfolinkResin Pierce for the US company's products, Freund's complete adjuvant, Freund's incomplete adjuvant Sigma, USA.

[0025] NaCl, KCl, Na 2 HPO 4 12h 2 O, kh 2 PO 4 , Tris, Glycine, EDTA, TMB, DMSO, analytical grade reagents such as glutaraldehyde.

[0026] 2, supplies:

[0027] Viskase dialysis bag for the US company's products, molecular weight cutoff 14kD.

[0028] P-10 column for the United States BioRad the company's products.

[0029] 3, the instrument:

[0030] Magnetic st...

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Abstract

The invention provides an immunogen for obtaining an Nrf1D protein antibody, the Nrf1D protein antibody and an Elisa detection kit. The immunogen is obtained as follows: polypeptide with the amino acid sequence shown in SEQ ID NO.1 is synthesized firstly, Sulfo-SMCC is used as a coupling agent, the polypeptide and KLH (keyhole limpet hemocyanin) are coupled; the antibody is obtained as follows: immunogen is taken as an antigen, the antigen is emulsified with FCA (freund's complete adjuvant) to immunize a New Zealand white rabbit, blood of the immunized New Zealand white rabbit is centrifuged, and a supernatant after centrifugation is collected; the Elisa detection kit comprises components including an ELISA plate, an enveloping solution, a blocking solution, an ELISA secondary antibody, a TMB color developing solution, a stop solution, a detection antigen and the Nrf1D protein antibody, the detection antigen is the polypeptide with the amino acid sequence shown in SEQ ID NO.1, glutaradehyde is used as a coupling agent, and the polypeptide and BSA bovine serum albumin are coupled.

Description

Technical field [0001] Technical Field The present invention belongs to an antibody, particularly antibodies directed Nrf1D for obtaining immunogens, antibodies and Nrf1D Elisa detection kit. Background technique [0002] CNC-bZIP is a subfamily bZIP transcription factors, which to a subfamily of about 40 amino acid homology domain of its characteristics, a basic N-terminal region of the leucine zipper region immediately. From an evolutionary point of view, having the homeodomain proteins in Caenorhabditis elegans (Skn1), Drosophila melanogaster (cap'n'collar), chicken (ECH) [63], mouse (Bach) and human During development, there are broad participation. In humans, three members (NF-E2, TCF11 / LCR-F1 / Nrf1 and Nrf2) currently known, and they have been shown to result in spectrum β-globin gene locus in vivo murine homolog It was isolated and identified in the process-specific expression mechanism. However, only NF-E2 only cell type specificity, Nrf1 and Nrf2 did not. Knockout mic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C07K16/18G01N33/68
CPCC07K14/00C07K14/795C07K16/18C07K19/00G01N33/6827G01N2333/46
Inventor 张义国汪红霞
Owner CHONGQING UNIV
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