Detection reagent and test paper for beta-hydroxybutyrate

A technology for detecting reagents and test strips, which is used in measuring devices, material analysis, instruments and other directions by observing the impact on chemical indicators, can solve problems such as insufficient detection speed, and achieve fast and accurate detection, rapid detection, and accurate detection. Effect

Inactive Publication Date: 2017-05-24
复星诊断科技(长沙)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the above existing technologies, the detection accur

Method used

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  • Detection reagent and test paper for beta-hydroxybutyrate
  • Detection reagent and test paper for beta-hydroxybutyrate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Step 1: Paste double-sided adhesive tape 40 on the reactive base layer 30 made of PET with a certain hardness, and punch holes for later use.

[0054] Step 2: tear off the double-sided adhesive tape on the reaction base layer 40, and paste the reaction film 50 on the double-sided tape 40 of the PET board. The reaction film 50 needs to completely cover the hole 31 on the PET substrate.

[0055] Step 3. Prepare the detection reagent according to the ratio: 3KU / L diaphorase, 9KU / L β-hydroxybutyrate dehydrogenase, 1.2KU / L NAD and 0.6KU / L NBT, 0.08mmol / L L (pH 8.0) Tris-HCl buffer solution, 1.0 g / L g / L trehalose. Add the prepared detection reagent dropwise into the wells of the reaction membrane plate with a dispenser, the weight of each hole is 6mg, and put the membrane plate after dripping into the drying tunnel for drying. The drying temperature is 37°C and the drying time is 20min, and the reaction is obtained. Layer 50.

[0056] Step 4: Paste and completely cover the ...

Embodiment 2

[0059] In this example, the detection reagent contains 3KU / L of diaphorase, 13KU / L of β-hydroxybutyrate dehydrogenase, 2.5KU / L of NAD and 0.6KU / L of NBT, 0.12mmol / L (pH 9.0 ) Tris-HCl buffer solution, 1.5g / L g / L trehalose, and others are the same as in Example 1.

Embodiment 3

[0061] In this example, the detection reagent contains 5KU / L diaphorase, 9KU / L β-hydroxybutyrate dehydrogenase, 1.2KU / L NAD and 1.0KU / L NBT, 0.08mmol / L (pH 8.0) Tris-HCl buffer solution, 1.0g / L g / L trehalose, and others are the same as in Example 1.

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Abstract

The invention discloses a detection reagent and test paper for beta-hydroxybutyrate. The detection reagent contains 3-5 KU/L of diaphorase, 9-13 KU/L of beta-hydroxybutyrate dehydrogenase, 1.2-2.5 KU/L of NAD and 0.6-1.0 KU/L of NBT. The detection reagent contains various specific enzymes for specific proportioning, thereby being capable of realizing rapid detection and more accurate detection. The test paper comprises a reaction base layer, a reaction layer, a blood filter layer and a hydrophilic layer which are stacked to form a siphon system, and rapid detection and more accurate detection are further guaranteed.

Description

technical field [0001] The invention relates to the technical field of beta-hydroxybutyric acid detection, in particular to a detection reagent for beta-hydroxybutyric acid and a detection test paper for beta-hydroxybutyric acid. Background technique [0002] Currently, methods for detecting the presence or amount of a certain component in blood include liquid reagent testing and dry chemical test strip testing. Dry chemical test paper has the characteristics of convenient use, simple operation and quick results. [0003] According to the flow path of the liquid sample on the dry chemical test paper, the dry chemical test paper can be divided into various types, and the vertical vertical detection test paper is a kind of dry chemical test paper. The vertical vertical detection test paper includes stacking the carriers with the reaction reagents on top of each other, the liquid sample flows through each reaction reagent layer from top to bottom, and finally displays the dete...

Claims

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Application Information

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IPC IPC(8): G01N21/77
CPCG01N21/77G01N2021/775G01N2021/7759
Inventor 彭勇李文韬李小慧
Owner 复星诊断科技(长沙)有限公司
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