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Method for determining content of six ginsenoside ingredients of folium ginseng

A technology of ginsenoside and determination method, which can be applied in the directions of measurement device, material separation, analysis material, etc., and can solve problems such as toxic and side effects

Inactive Publication Date: 2017-05-24
河北省药品检验研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although ginseng leaves are good, they should not be abused. As the efficacy of ginseng leaves has gradually become known to people, in recent years, they have gradually become abused, and even caused a variety of toxic side effects.

Method used

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  • Method for determining content of six ginsenoside ingredients of folium ginseng
  • Method for determining content of six ginsenoside ingredients of folium ginseng
  • Method for determining content of six ginsenoside ingredients of folium ginseng

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Chromatographic conditions and system suitability test The chromatographic column uses octadecylsilane bonded silica gel as a micro-column; acetonitrile is used as mobile phase A, water is used as mobile phase B, and gradient elution is carried out. The elution gradient is: 0 ~9 minutes, the mobile phase A gradually changes from 18% to 20%; 9~13 minutes, the mobile phase A gradually changes from 20% to 28%; 13~28 minutes, the mobile phase A gradually changes from 28% to 35%; the detection wavelength is 203nm; flow rate 0.4ml / min;

[0088] Preparation of mixed reference solution

[0089] Weigh ginsenoside Rg 1 Reference substance, ginsenoside Re reference substance, ginsenoside Rb 1 Reference substance, ginsenoside Rb 2 Appropriate amount of reference substance, 20(S)-ginsenoside F1 reference substance, ginsenoside Rd reference substance, accurately weighed, and made by adding methanol. Each 1ml contains 100μg of ginsenoside Rg1, 500μg of ginsenoside Re, and ginsenosi...

Embodiment 2

[0093] Chromatographic conditions and system suitability test The chromatographic column uses octadecylsilane bonded silica gel as a micro-column; acetonitrile is used as mobile phase A, water is used as mobile phase B, and gradient elution is carried out. The elution gradient is: 0 ~9 minutes, the mobile phase A gradually changes from 18% to 20%; 9 to 13 minutes, the mobile phase A gradually changes from 20% to 28%; 13 to 28 minutes, the mobile phase A gradually changes from 28% to 35%; the detection wavelength is 203nm; flow rate 0.4ml / min;

[0094] Preparation of mixed reference solution

[0095] Weigh ginsenoside Rg 1 Reference substance, ginsenoside Re reference substance, ginsenoside Rb 1 Reference substance, ginsenoside Rb 2 Appropriate amount of reference substance, 20(S)-ginsenoside F1 reference substance, and ginsenoside Rd reference substance, accurately weighed, and made by adding methanol, each 1ml contains ginsenoside Rg 1 100μg, Ginsenoside Re500μg, Ginsenos...

Embodiment 3

[0099] Chromatographic conditions and system suitability test The chromatographic column uses octadecylsilane bonded silica gel as a micro-channel column; acetonitrile is used as mobile phase A, water is used as mobile phase B, and gradient elution is carried out. The elution gradient is: 0 ~9 minutes, mobile phase A gradually changes from 18% to 20%; 9 ~ 13 minutes, mobile phase A gradually changes from 20% to 28%; 13 ~ 28 minutes, mobile phase A gradually changes from 28% to 35%; detection wavelength is 203nm; flow rate 0.4ml / min;

[0100] Preparation of mixed reference solution

[0101] Weigh ginsenoside Rg 1 Reference substance, ginsenoside Re reference substance, ginsenoside Rb 1 Reference substance, ginsenoside Rb 2 Appropriate amount of reference substance, 20(S)-ginsenoside F1 reference substance, and ginsenoside Rd reference substance, accurately weighed, and made by adding methanol, each 1ml contains ginsenoside Rg 1 100μg, Ginsenoside Re500μg, Ginsenoside Rb 1 ...

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Abstract

The invention provides a method for determining content of six ginsenoside ingredients of folium ginseng. Ginsenoside is the main active ingredient of the folium ginseng and an important criterion for measuring the quality of the folium ginseng, but the content is greatly different from that of ginseng. According to the method, ultra-high performance liquid chromatography is used for determining the six ginsenoside ingredients including ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rb2, 20(S)-ginsenoside F1 and ginsenoside Rd in the folium ginseng simultaneously, the method is high in sensitivity and accurate, and can be used for quality control of the folium ginseng.

Description

technical field [0001] The invention relates to a content determination method of ginsenoside components in ginseng leaves, which belongs to the technical field of traditional Chinese medicine, and specifically relates to a ginsenoside Rg in ginseng leaves 1 , Ginsenoside Re, Ginsenoside Rb 1 , Ginsenoside Rb 2 , 20(S)-ginsenoside F 1 and ginsenoside Rd six components of the simultaneous determination of the content determination method. Background technique [0002] Ginseng leaves are the dried leaves of Araliaceae plant Panax ginseng C.A.Mey. Harvested in autumn, dried or dried. Ginseng leaves are bitter, sweet, and cold, and return to the two meridians of the lung and stomach. It was first recorded in "Compendium of Materia Medica Supplements". , benefits the limbs and heads", "promoting fluid and moistening dryness, benefiting the lungs and liver, and nourishing vitality" and other functions. Modern studies have shown that the pharmacological effects of ginseng leav...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 赵振霞刘永利雷蓉王敏李冬梅
Owner 河北省药品检验研究院
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