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Method for rapidly removing potato virus

A potato virus removal technology, applied in horticultural methods, botanical equipment and methods, disinfectants, etc., can solve the problems of plant poisoning, long time, and long cultivation time of tissue culture seedlings, and achieve low virus infection rate and inhibition Reproducible, easy-to-produce effects

Active Publication Date: 2017-05-31
云南省农业科学院生物技术与种质资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of ribavirin will cause toxicity to the plant itself, even at very low concentrations, the growth and development of the plant will be seriously affected
Adding ribavirin in the medium will make the culture time of tissue culture seedlings too long, even if it can be detoxified, it will take a long time, which is not conducive to obtaining non-toxic seed potatoes in time in production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The potato variety is cooperation 88, the main variety cultivated in Yunnan in recent years. The potato sample was detected by electron microscope negative staining and ELISA. Potato virus S, potato virus Y, potato virus A, potato leafroll virus, potato M virus, Virus.

[0021] Material processing: select potato cubes with moderate size and smoothness, after 7-15 days of germination, cut off the stem tip of the germination-treated potato cubes by 0.5-1 cm, rinse with clean water, and then carry out strict disinfection on the ultra-clean workbench. Soak in 75% alcohol for 15 seconds, wash twice with sterile water, then soak with 0.1% mercury liter for 3 to 5 minutes, rinse with sterile water for 3 to 5 times, each time for 3 minutes, and absorb it with sterilized absorbent paper Dry, inoculated in Ms medium and cultured for 7 days before use.

[0022] Inducer treatment: use inducer: 3-acetonyl-3-hydroxyoxindole (3-acetonyl-3-hydroxyoxindole, AHO) at a concentration of 2...

Embodiment 2

[0027] The potato variety is Haoshu 302, the main cultivar in Zhaotong, Yunnan. Negative staining by electron microscopy and ELISA detected that the potato sample contained potato S virus.

[0028] Material processing: select potato cubes with moderate size and smoothness, after 7-15 days of germination, cut off the stem tip of the germination-treated potato cubes by 0.5-1 cm, rinse with clean water, and then carry out strict disinfection on the ultra-clean workbench. Soak in 75% alcohol for 15 seconds, wash twice with sterile water, then soak with 0.1% mercury liter for 3 to 5 minutes, rinse with sterile water for 3 to 5 times, each time for 3 minutes, and absorb it with sterilized absorbent paper Dry, inoculated in Ms medium and cultured for 7 days before use.

[0029] Inducer treatment: use inducer: 3-acetonyl-3-hydroxyoxindole (3-acetonyl-3-hydroxyoxindole, AHO) at a concentration of 2-20 micrograms / ml to directly spray on the leaf surface of tissue cultured potato, Spray...

Embodiment 3

[0034] Potato varieties are Jingshu No. 3 and No. 4, the main cultivars in Qujing, Yunnan. Negative staining by electron microscope and ELISA detected that the potato samples contained potato virus Y and potato leafroll virus.

[0035] Material processing: select potato cubes with moderate size and smoothness, after 7-15 days of germination, cut off the stem tip of the germination-treated potato cubes by 0.5-1 cm, rinse with clean water, and then carry out strict disinfection on the ultra-clean workbench. Soak in 75% alcohol for 15 seconds, wash twice with sterile water, then soak with 0.1% mercury liter for 3 to 5 minutes, rinse with sterile water for 3 to 5 times, each time for 3 minutes, and absorb it with sterilized absorbent paper Dry, inoculated in Ms medium and cultured for 7 days before use.

[0036] Inducer treatment: use inducer: Ningnanmycin concentration of 60-80 micrograms / ml to directly spray on the leaf surface of tissue-cultured potato, and spray once 3-5 days ...

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PUM

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Abstract

The invention discloses a method for rapidly removing a potato virus. The method comprises the steps that a plant resistance inducer for inhibiting the virus is sprayed on a tissue culture seedling at 3-5 days before stem tip peeling based on conventional stem tip detoxication, wherein the plant resistance inducer includes but is not limited to amino-oligosaccharin, ningnanmycin, 3-hydroxyl-3-acetonyl ketoindole, and the like; a stem tip is taken and separately cultured for 7-28 days; the plant resistance inducer for inhibiting the virus is sprayed for one time; 0.1-0.7mm stem tip is taken and separately cultured for 7-28 days; and the step is repeated for three times to obtain a virus-free tissue culture seedling. A virus-free rate of the obtained tissue culture seedling is higher than 80%. According to the method, the time for obtaining the virus-free tissue culture seedling is short; a virus rate of the obtained tissue culture seedling is low; operation is simple; and production popularization is facilitated.

Description

technical field [0001] The invention belongs to the technical field of plant disease prevention and control, and in particular relates to a method for quickly removing potato viruses. Background technique [0002] Potato is an important crop that is gradually becoming a staple food in my country. Potato seed potato reproduction mainly relies on asexual reproduction, which is very susceptible to virus infection. Potatoes infected with the virus have significantly reduced yield and quality. Potato virus disease has become an important damage to the potato industry. The main method of preventing and controlling potato virus disease is to detoxify the shoot tip. However, the traditional shoot tip detoxification takes a long time to obtain virus-free seedlings. Some viruses are difficult to remove after repeated peeling of the shoot tip, such as potato S virus. In the early stage, there was also information disclosing the use of ribavirin to interfere with virus replication to...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01C1/08A01G7/06A01N43/54A01N43/38A01N43/16A01P1/00A01P21/00
CPCA01C1/08A01G7/06A01H4/008A01N43/16A01N43/38A01N43/54A01N2300/00
Inventor 董家红张丽珍吴阔赵立华陈永对苏晓霞郑宽瑜张仲凯
Owner 云南省农业科学院生物技术与种质资源研究所
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