Method for degrading deoxynivalenol
A technology for deoxynivalenol and crops is applied in the field of crop detection to achieve the effects of protecting the health of consumers, having broad market prospects and protecting the growth of livestock, poultry and animals.
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Embodiment 1
[0018] 1. Materials and methods
[0019] 1.1 Main equipment and reagents
[0020] 10Mev electron accelerator irradiation device (Shanghai Shuneng Irradiation Technology Co., Ltd.), high performance liquid chromatography (US Waters Company), vortex instrument (Haimen Qilin Bell Company), nitrogen blowing instrument (Xi'an Jingda Testing Equipment Co., Ltd. ), Milli-Q ultrapure water machine (Millipore, Germany).
[0021] 1.2 Standards and reagents
[0022] Mycotoxin DON standard substance (≥99%, HPLC), ROMER International Trading (Beijing) Co., Ltd.; methanol and acetonitrile were chromatographically pure, purchased from MERCK, Germany; deoxynivalenol immunoaffinity column, ROMER International Trading (Beijing) Co., Ltd.; other reagents were purchased from Sinopharm Chemical Reagent Co., Ltd.
[0023] 2. Experimental method
[0024] 2.1 Solution preparation
[0025] DON standard solutions were prepared with acetonitrile aqueous solution (acetonitrile+water=84+16), the conc...
Embodiment 2
[0038] 1. Materials and methods
[0039] Wheat was purchased from Yancheng City, Jiangsu Province, with an initial DON content of 1400 μg / kg. Other materials and reagents are identical to Example 1.
[0040] 2. Experimental method
[0041] 2.1 Wheat sample preparation
[0042] The wheat samples are not subjected to any treatment, and the wheat grains are directly placed in iron flat-bottomed trays, spread flat, the thickness of which does not exceed 8cm, and the mass of each sample is 25kg, which is to be irradiated.
[0043] 2.2 Irradiation treatment
[0044] Put the 2.1 wheat sample on the assembly line of the 10MeV electron accelerator irradiation device, set the irradiation doses to 2, 4, 6, 8, 10, 12, 14, 16, 18 and 20kGy respectively, and repeat each irradiation dose 3 times , and set the unirradiated sample as the control group.
[0045] 2.3 DON concentration determination is the same as in Example 1.
[0046] The degradation rate is calculated according to the fo...
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