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Method for degrading deoxynivalenol

A technology for deoxynivalenol and crops is applied in the field of crop detection to achieve the effects of protecting the health of consumers, having broad market prospects and protecting the growth of livestock, poultry and animals.

Inactive Publication Date: 2017-05-31
SHANGHAI CO ELITE AGRI PRUDUCTS INSPECTION TECH SERVICE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, rolling, shelling, residue removal, water washing, acid valency, etc. can also reduce mycotoxins in food and feed, but the above-mentioned treatment methods have certain defects

Method used

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  • Method for degrading deoxynivalenol
  • Method for degrading deoxynivalenol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Materials and methods

[0019] 1.1 Main equipment and reagents

[0020] 10Mev electron accelerator irradiation device (Shanghai Shuneng Irradiation Technology Co., Ltd.), high performance liquid chromatography (US Waters Company), vortex instrument (Haimen Qilin Bell Company), nitrogen blowing instrument (Xi'an Jingda Testing Equipment Co., Ltd. ), Milli-Q ultrapure water machine (Millipore, Germany).

[0021] 1.2 Standards and reagents

[0022] Mycotoxin DON standard substance (≥99%, HPLC), ROMER International Trading (Beijing) Co., Ltd.; methanol and acetonitrile were chromatographically pure, purchased from MERCK, Germany; deoxynivalenol immunoaffinity column, ROMER International Trading (Beijing) Co., Ltd.; other reagents were purchased from Sinopharm Chemical Reagent Co., Ltd.

[0023] 2. Experimental method

[0024] 2.1 Solution preparation

[0025] DON standard solutions were prepared with acetonitrile aqueous solution (acetonitrile+water=84+16), the conc...

Embodiment 2

[0038] 1. Materials and methods

[0039] Wheat was purchased from Yancheng City, Jiangsu Province, with an initial DON content of 1400 μg / kg. Other materials and reagents are identical to Example 1.

[0040] 2. Experimental method

[0041] 2.1 Wheat sample preparation

[0042] The wheat samples are not subjected to any treatment, and the wheat grains are directly placed in iron flat-bottomed trays, spread flat, the thickness of which does not exceed 8cm, and the mass of each sample is 25kg, which is to be irradiated.

[0043] 2.2 Irradiation treatment

[0044] Put the 2.1 wheat sample on the assembly line of the 10MeV electron accelerator irradiation device, set the irradiation doses to 2, 4, 6, 8, 10, 12, 14, 16, 18 and 20kGy respectively, and repeat each irradiation dose 3 times , and set the unirradiated sample as the control group.

[0045] 2.3 DON concentration determination is the same as in Example 1.

[0046] The degradation rate is calculated according to the fo...

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Abstract

The invention relates to a method for degrading deoxynivalenol. The method specifically comprises the following steps: spreading crops, putting on a 10Mev electron accelerator irradiation device production line, and setting the irradiation dose as 2-20 kGy. The method provided by the invention has the advantages of simple and quick treatment, high degradation rate and the like, and is applicable to degradation, transformation and removal of fungaltoxin DON in crops and foods, thereby providing effective reference and technical support for quality safety guarantee of grains and feeds in China.

Description

technical field [0001] The invention relates to the field of crop detection, in particular to a method for degrading deoxynivalenol. Background technique [0002] Mycotoxin is a secondary metabolite produced by fungi, and its pollution in crop products, food and feed is becoming more and more serious. Among them, type B trichothecenes produced by Fusarium fungi are the most common mycotoxins in the world. It widely contaminates wheat, barley, corn and other cereals and their products. It is estimated that 25% of the world's food crops are contaminated with mycotoxins every year. According to the estimates of the Food and Agriculture Organization of the United Nations (FAO), the economic losses caused by this worldwide are hundreds of billions of dollars every year. The Jianghuai region of my country is one of the main producing areas of wheat, corn and other crops, and the mycotoxin pollution of wheat and corn is relatively serious. The detection rate of mycotoxin deoxyn...

Claims

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Application Information

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IPC IPC(8): A23L5/20
Inventor 杨宪立周昌艳赵志勇聂冬霞赵晓燕陈珊珊周佳欣姚春霞李晓贝范婷婷陈磊
Owner SHANGHAI CO ELITE AGRI PRUDUCTS INSPECTION TECH SERVICE CO LTD