Individualized formula food for immune regulation ability-related gene and preparation method of individualized formula food

A formula food and immune regulation technology, applied in biochemical equipment and methods, food ingredients, pharmaceutical formulations, etc., can solve the problem of impaired regulation of immune cell activity, abnormal immune tolerance of T cells, and influence on the expression level of inflammatory factors, etc. to improve and maintain normal intestinal function, reduce the burden on the liver, and improve immunity

Inactive Publication Date: 2017-05-31
上海中优医学检验所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Genes related to immune regulation ability include the gene encoding interlein 1β receptor (IL1β), the gene encoding cytolytic T lymphocyte-associated antigen 4 (CTLA4), and the gene encoding tumor necrosis factor-α (TNF-α). The mutation affects the expression level of inflammatory factors, and the local excessive and / or reduced production of inflammatory factors increases the risk of cancer; CTLA4 gene mutations lead to abnormal immune tolerance of T cells and increase the risk of various diseases such as tumors; TNF -A gene mutation leads to impaired regulation of immune cell activity in the body and increased susceptibility to various diseases

Method used

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  • Individualized formula food for immune regulation ability-related gene and preparation method of individualized formula food
  • Individualized formula food for immune regulation ability-related gene and preparation method of individualized formula food
  • Individualized formula food for immune regulation ability-related gene and preparation method of individualized formula food

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Individual oral epithelial cell samples were collected, and genomic DNA was extracted by silica gel adsorption method. After electrophoresis detection and verification, fluorescent quantitative PCR reaction was performed.

[0076] Put individual samples into 3 reaction wells, and detect 3 sites at the same time, that is, site 1 of IL1β gene: rs1143627; site 2 of CTLA4 gene: rs4553808; site 3 of TNF-α gene: rs1800629. In addition, NTC blank control wells without DNA templates were added according to the experimental needs;

[0077] Add reagents to each reaction well as a fluorescent quantitative PCR reaction system, with a total volume of 10 μL, that is, 2 μL of DNA template with a concentration of 20 ng / μL, 10× fluorescent quantitative PCR reaction buffer MGB 1μL, 25mM dNTP Four deoxynucleotide substrates for DNA synthesis 0.1μL, 25mM MgCl 2 Solution 0.5μL, (5units / μL) TaqDNA polymerase 0.02μL, deionized water 4.98μL, 3 sites respectively using different forward prime...

Embodiment 2

[0084] According to the relevant research results of molecular epidemiology in a large-scale Chinese population, the risk of different genotypes of the three loci measured in the embodiment is shown in Table 2 below:

[0085] Table 2 Risk degree without genotype

[0086]

[0087] There are three subtypes for each gene polymorphism site related to immune regulation ability, and the possible permutations and combinations of all subtypes at the selected site; then the risk of these subtypes is multiplied to obtain the risk of different permutations and combinations Risk product; arrange the obtained risk products from small to large or from large to small, and merge groups with equal risk products; finally determine the level of risk of each group according to the size of the risk product.

[0088] The above method weights each site as an independent factor, and the greater the risk product, the greater the estimated risk; There are many genetic risk factors that cause tumors...

Embodiment 3

[0098] Prepare the necessary raw materials for the basic formula: 3 parts of pea protein, 1 part of chitosan, 0.5 part of mung bean starch, 0.5 part of whey protein, and 0.5 part of wheatgrass.

[0099] Supplementary raw materials for the basic formula: 2 parts of rice protein, 2 parts of kudzu root powder, 0.5 part of probiotics, and 0.5 part of prebiotics.

[0100] Necessary raw materials for genetic formula: 2 parts of epimedium, 1 part of astragalus polysaccharide, 2 parts of glutamine, 1 part of arginine, 1 part of leucine, 0.5 part of zinc.

[0101] Supplementary raw materials for Gene Formula: 1 part of glycyrrhizin, 1 part of Ganoderma lucidum polysaccharide, 0.5 part of n-3 fatty acid, and 0.5 part of selenium.

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Abstract

The invention provides an individualized formula food for an immune regulation ability-related gene. The individualized intervention formula food is prepared through the following steps: (1) detecting variations of an interleukin 1beta receptor coding gene, a cell solubility T lymphocyte-related antigen-4 coding gene and a tumor necrosis factor-alpha coding gene of an individual; (2) evaluating the variation risks of the detected genes; and (3) selecting raw materials in a basic formula of the food, selecting raw materials in gene formulas according to the evaluated risks and preparing the individualized formula food for the immune regulation ability-related gene. According to the individualized intervention formula food, the immunity of a tumor patient can be improved.

Description

technical field [0001] The invention relates to an individualized intervention formula food, in particular to an individualized formula food for genes related to immune regulation ability. Background technique [0002] According to the latest data from the "2012 China Tumor Registration Annual Report", there are about 3.12 million new cases of malignant tumors in my country every year, and 6 people are diagnosed with malignant tumors every minute; about 2.7 million deaths due to malignant tumors are made every year. Every minute, 5 people die of malignant tumors. Cancer patients are prone to malnutrition because of their special metabolic conditions, tumor cells need more nutrition, and the discomfort caused by surgery, radiotherapy and chemotherapy aggravates the patient's anorexia. Malnutrition will lead to weight loss, decreased immunity, and reduced tolerance of patients, which will greatly shorten the survival time. If the nutrition problem is neglected and the timing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/00A23L33/185A23L33/125A23L33/26A23L33/19A23L33/10A23L33/135A23L33/175A23L33/16A23L33/12A23L19/10A23L33/105A61K38/17A61K36/296A61K33/04A61P3/02A61P37/04C12Q1/68A61K31/722A61K31/718A61K35/741A61K31/715A61K31/198A61K33/30A61K31/704A61K31/202
CPCA23V2002/00A61K31/198A61K31/201A61K31/704A61K31/715A61K31/718A61K31/722A61K33/04A61K33/30A61K35/741A61K36/296A61K36/488A61K36/899A61K38/168A61K38/1709C12Q1/6851A23V2200/324A23V2200/30A23V2200/308A23V2250/0606A23V2250/062A23V2250/0628A23V2250/1626A23V2250/1642A23V2250/186A23V2250/252A23V2250/51A23V2250/5118A23V2250/511A23V2250/54252A23V2250/5482A23V2250/5488A61K2300/00C12Q2531/113C12Q2563/107
Inventor 王校毛丹丹张奕傅咏南陆婧黄芬芬卞雪莲
Owner 上海中优医学检验所有限公司
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