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A kind of extraction method of mannotriose in fresh rehmannia glutinosa

A technology of mannotriose and extraction method, which is applied in the field of extracting mannotriose in fresh rehmannia glutinosa, can solve the problems of low extraction rate and high energy consumption, and achieve the effects of simple preparation process, reduced process steps and rich content

Inactive Publication Date: 2019-07-09
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the above problems of high energy consumption and low extraction rate in the process of extracting mannotriose from fresh rehmannia glutinosa in the prior art, the present application discloses a method for extracting mannotriose from fresh rehmannia glutinosa to extraction and purification. method

Method used

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  • A kind of extraction method of mannotriose in fresh rehmannia glutinosa
  • A kind of extraction method of mannotriose in fresh rehmannia glutinosa
  • A kind of extraction method of mannotriose in fresh rehmannia glutinosa

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Embodiment 1

[0038] Embodiment 1 A method for preparing high-purity mannotriose from fresh rehmannia glutinosa, consisting of the following steps:

[0039] (1) High-temperature processing of fresh rehmannia glutinosa: take 25.0 g of fresh rehmannia glutinosa after harvesting, discard the rotten parts, wash and slice, cut into slices with a thickness of 3 cm, heat in airtight to 90°C for 6 hours;

[0040] (2) Preparation of mannotriose sample liquid: add the processed fresh rehmannia glutinosa obtained in step (1) into water at 70°C for 0.5 h at a mass ratio of 1:2, and continue to extract the residue once according to the above process , combined extracts, vacuum concentrated to 60 ° Brix sugar solution, that is, to obtain mannotriose sample liquid.

[0041] (3) Alcohol precipitation to obtain mannotriose sample solution Alcohol precipitation part: add ethanol to the mannotriose sample solution at a ratio of 1:2 by volume, keep it warm at 80°C for 30 minutes, cool down naturally for 24 hou...

Embodiment 2

[0044] Embodiment 2 A method for preparing high-purity mannotriose from fresh rehmannia glutinosa, consisting of the following steps:

[0045] (1) High-temperature processing of fresh rehmannia glutinosa: take 50.0 g of fresh rehmannia glutinosa after harvesting, discard the rotten parts, wash and slice, cut into slices with a thickness of 4 cm, heat in airtight to 95°C for 5 hours;

[0046] (2) Mannotriose sample liquid preparation: add the fresh rehmannia glutinosa processed product obtained in step (1) into water at 80°C for 1 hour at a mass ratio of 1:2.5, and continue to extract the residue once according to the above process. The extracts were combined and concentrated in vacuo to a 60°Brix sugar solution to obtain a mannotriose-like solution.

[0047] (3) Alcohol precipitation to obtain mannotriose sample solution Alcohol precipitation part: add ethanol to mannotriose sample solution at a ratio of 1:2 by volume, keep it warm at 80°C for 30 minutes, cool down naturally f...

Embodiment 3

[0050] Embodiment 3 A method for preparing high-purity mannotriose from fresh rehmannia glutinosa, consisting of the following steps:

[0051] (1) High-temperature processing of fresh rehmannia glutinosa: take 100.0 g of fresh rehmannia glutinosa after harvesting, discard the rotten parts, wash and slice, cut into slices with a thickness of 3 cm, heat in airtight to 90°C for 6 hours;

[0052] (2) Preparation of mannotriose sample liquid: add the processed fresh rehmannia glutinosa obtained in step (1) into water at 80°C for 1 hour at a mass ratio of 1:2, and continue to extract the residue once according to the above process. The extracts were combined and concentrated in vacuo to a sugar solution of 60°Brix to obtain a mannotriose-like solution.

[0053](3) Alcohol precipitation to obtain mannotriose sample solution Alcohol precipitation part: add ethanol to the mannotriose sample solution at a ratio of 1:2 by volume, keep it warm at 80°C for 30 minutes, cool down naturally f...

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Abstract

The invention relates to the technical field of mannotriose extraction and preparation, in particular to a method for extracting mannotriose from fresh rehmannia glutinosa: fresh rehmannia glutinosa is processed at high temperature; mannotriose sample liquid is prepared; alcohol precipitation; impurity removal, decolorization and concentration; chromatographic separation. The high-temperature processing of fresh rehmannia directly without the operation of raw rehmannia greatly reduces the processing steps of fresh rehmannia, and, from the yield data, it also has a positive impact; the temperature during water extraction is 70- 80 ℃, instead of the usual boiling water extraction, has a significant impact on improving the yield; after steaming at high temperature, the resin and gel are combined to obtain mannotriose, with a total yield of more than 4.0% and a purity of more than 98.0% , providing an environmentally friendly, convenient and economical method for the preparation of high-purity mannotriose.

Description

technical field [0001] The invention relates to the technical field of mannotriose extraction and preparation, in particular to a method for extracting mannotriose in fresh rehmannia glutinosa. Background technique [0002] Mannotriose (English name: manninotriose, molecular formula C 18 h 32 o 16 ), is a natural functional oligosaccharide, which can effectively proliferate beneficial bacteria (bifidobacteria and lactic acid bacteria, etc.) in the human intestinal tract, and has good physiological functions. Most importantly, mannotriose is the chemical component with the highest content after processing and processing mature rehmannia glutinosa from fresh rehmannia glutinosa, and the content is as high as 30% (on a dry basis). The method of preparing high-purity mannotriose is expected to make mannotriose into a class of innovative drugs through further research and development, and provide reference for the quality control of rehmannia glutinosa. [0003] [0004] C...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H3/06C07H1/08
CPCC07H1/08C07H3/06
Inventor 高辉胡德福王维剑凌霄徐玉文牛冲陈真王文心
Owner SHANDONG INST FOR FOOD & DRUG CONTROL