Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sepia pharaonis neuropeptide GnPH procreation regulation preparation

A technology of tiger squid and neuropeptide, which is applied in the field of tiger squid neuropeptide GnRH reproductive regulation preparation, can solve the problems of tissue preservation and other issues that need to be improved, and achieve the effect of preventing tissue loss of water and activity, preventing tissue degradation, and excellent effect

Inactive Publication Date: 2017-05-31
ZHEJIANG OCEAN UNIV
View PDF3 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention has the advantages of simple sequence and strong analgesic activity, but the early tissue preservation needs to be improved, and the extraction process still has room for improvement

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Tiger squid neuropeptide GnRH reproductive regulation preparation, the preparation steps are as follows:

[0022] 1) Pretreatment: Dissect the living tiger squid, take the brain tissue in the preservation solution, and store it at ultra-low temperature;

[0023] 2) Take the supernatant: Take the brain tissue and add an equal volume of normal saline, homogenate, centrifuge, take the supernatant A, then add an equal volume of distilled water, centrifuge, take the supernatant B, combine the supernatants A and B to get the above Serum C;

[0024] 3) Column chromatography: the supernatant C is subjected to column chromatography, chromatography, color development, and components are collected to obtain neuropeptides;

[0025] 4) Catalysis: Under the catalysis of ammonium nitrate and metal cadmium, the neuropeptide undergoes a substitution reaction with a GnRH derivative to generate a tiger squid neuropeptide GnRH reproductive regulator.

[0026] In step 1, the ultra-low tem...

Embodiment 2

[0033] Tiger squid neuropeptide GnRH reproductive regulation preparation, the preparation steps are as follows:

[0034] 1) Pretreatment: Dissect the living tiger squid, take the brain tissue in the preservation solution, and store it at ultra-low temperature;

[0035] 2) Take the supernatant: Take the brain tissue and add an equal volume of normal saline, homogenate, centrifuge, take the supernatant A, then add an equal volume of distilled water, centrifuge, take the supernatant B, combine the supernatants A and B to get the above Serum C;

[0036] 3) Column chromatography: the supernatant C is subjected to column chromatography, chromatography, color development, and components are collected to obtain neuropeptides;

[0037] 4) Catalysis: Under the catalysis of ammonium nitrate and metal cadmium, the neuropeptide undergoes a substitution reaction with a GnRH derivative to generate a tiger squid neuropeptide GnRH reproductive regulator.

[0038] In step 1, the cryopreservat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a sepia pharaonis neuropeptide GnPH procreation regulation preparation. The sepia pharaonis neuropeptide GnPH procreation regulation preparation is prepared by the steps: 1) pre-treatment: taking live sepia pharaonis, dissecting the live sepia pharaonis to take a brain tissue and preserving the brain tissue in a preservation solution at an ultra-low temperature; 2) taking of supernatant: taking the brain tissue, adding saline of the same volume to the brain tissue, homogenizing and centrifuging an obtained mixture to take supernatant A; adding distilled water of the same volume to the obtained mixture, centrifuging the mixture with the distilled water to take supernatant B; and combining the supernatant A and the supernatant B to obtain supernatant C; 3) column chromatography: carrying out column chromatography, chromatography and developing on the supernatant C and collecting components to obtain neuropeptide; and 4) catalysis: enabling the neuropeptide to have substitution reaction with a GnRH derivative under the catalysis of ammonium nitrate and cadmium metal to generate the sepia pharaonis neuropeptide GnPH procreation regulation preparation. The sepia pharaonis neuropeptide GnPH procreation regulation preparation, provided by the invention, has the advantages that the tissue can be preserved in a frozen manner and the preservation effect is excellent; the brain tissue degradation of the sepia pharaonis can be effectively prevented; the extraction rate of the neuropeptide is higher; the prepared control agent does not need to be continuously or repeatedly injected for a long time; and the preparation is applied to drugs for preventing or treating hormone-dependent diseases.

Description

technical field [0001] The invention belongs to the technical field of reproductive regulating preparations, and in particular relates to a reproductive regulating preparation of tiger squid neuropeptide GnRH. Background technique [0002] Tabby squid ( Sepia pharaonis ) are economically important cephalopods that occupy an important position in marine ecosystems. Its meat is fresh and delicious, rich in nutrients, and has potential medicinal value. Regular consumption plays an important role in improving the body's immunity. [0003] GnRH is an ancient and widely distributed reproductive hormone in animals. GnRH plays a very important role in the regulation of reproduction in animals. If the human body lacks GnRH, it will cause a series of body lesions, such as Kallmann Syndrome (KS). Hormonal hypogonadism. However, injecting GnRH or GnRH analogues to farmed animals can reduce the level of related hormones produced by the animal itself, and eventually lose all or part ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/435C07K1/14C07K1/16
Inventor 迟长凤孙连莲曹子豪吕振明徐梅英吴常文
Owner ZHEJIANG OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com