Method for extracting polysaccharides from artemisia rupestris

An extraction method, polysaccharide extraction technology, applied in the field of polysaccharide extraction, Xinjiang Artemisia polysaccharide extraction, can solve the problems of unsuitable for large-scale industrial production, complex process, low extraction rate, etc., to achieve accurate and reliable extraction conditions, simple process, and high extraction rate high effect

Inactive Publication Date: 2017-05-31
MINZU UNIVERSITY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing polysaccharide extraction techniques of Artemisia chinensis include water boiling method and microwave-assisted method, all of which have defects such as low extraction rate, complicated process, high cost, and unsuitable for industrialized large-scale production.

Method used

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  • Method for extracting polysaccharides from artemisia rupestris
  • Method for extracting polysaccharides from artemisia rupestris
  • Method for extracting polysaccharides from artemisia rupestris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Take Artemisia argyi from Xinjiang, place it in 95% ethanol with a solid-to-liquid ratio of 1:4 (g:mL), reflux twice in a hot water bath for 2 hours each time, and dry in the air.

[0023] (2) Pulverize the dried Artemisia chinensis in step (1), pass through a 40-mesh sieve, place in water, and use ultrasonic waves to assist in the extraction of Artemisia xinjiang polysaccharides, the ratio of solid to liquid is 1:30 (g:mL), and the extraction temperature The temperature is 72°C, the extraction time is 2.5h, and the ultrasonic power is 350W. After filtering the extract, concentrate it in a water bath at 80°C.

[0024] (3) Take the concentrated extract in step (2), add 4 times the concentration of the extract volume to 95% ethanol for alcohol precipitation, the alcohol precipitation time is 12h, and centrifuge at 4000r / min for 10min to obtain the precipitate and dry it to obtain Artemisia polysaccharides from Xinjiang.

[0025] (4) Deproteinization using HPD-300 dyn...

Embodiment 2

[0032] (1) Take Artemisia argyi from Xinjiang, place it in 95% ethanol with a solid-to-liquid ratio of 1:4 (g:mL), reflux twice in a hot water bath for 2 hours each time, and dry in the air.

[0033] (2) Pulverize the dried Artemisia chinensis in step (1), pass through a 40-mesh sieve, place in water, and use ultrasound to assist extraction of Artemisia xinjiang polysaccharides, the ratio of solid to liquid is 1:20 (g:mL), and the extraction temperature The temperature is 70°C, the extraction time is 2.5h, and the ultrasonic power is 350W. After filtering the extract, concentrate it in a water bath at 80°C.

[0034] (3) Take the concentrated extract in step (2), add 4 times the concentration of the extract volume to 95% ethanol for alcohol precipitation, the alcohol precipitation time is 12h, and centrifuge at 4000r / min for 10min to obtain the precipitate and dry it to obtain Artemisia polysaccharides from Xinjiang.

[0035] (4) Deproteinization using HPD-300 dynamic macropor...

Embodiment 3

[0038] (1) Take Artemisia argyi from Xinjiang, put it in 95% ethanol with a solid-to-liquid ratio of 1:5 (g:mL), reflux twice in a hot water bath for 2 hours each time, and dry in the air.

[0039] (2) Pulverize the dried Artemisia chinensis in step (1), pass through a 40-mesh sieve, place in water, and use ultrasonic waves to assist extraction of Artemisia sativa polysaccharides, the ratio of solid to liquid is 1:10 (g:mL), and the extraction temperature The temperature is 80°C, the extraction time is 2.5h, and the ultrasonic power is 350W. After the extract is filtered, it is concentrated in a water bath at 80°C.

[0040] (3) Take the concentrated extract in step (2), add 4 times the concentration of the extract volume to 95% ethanol for alcohol precipitation, the alcohol precipitation time is 12h, and centrifuge at 4000r / min for 10min to obtain the precipitate and dry it to obtain Artemisia polysaccharides from Xinjiang.

[0041](4) Utilize HPD-300 dynamic macroporous adso...

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Abstract

The invention relates to a method for extracting polysaccharides from artemisia rupestris. The method has the advantages that ultrasonic-assisted extraction technologies are adopted, proteins are removed by the aid of dynamic macroporous adsorption resin processes, accordingly, the method is high in extraction rate, low in cost and suitable for industrial mass production, the technologies are simple, the extraction rates of polysaccharide crude products from the artemisia rupestris are 3.11% at least, effects of removing more than 60% of hydroxyl free radicals and DPPH (1, 1-diphenyl-2-picyl-hydrazyl) can be realized when the concentration is 2.5 mg / mL, and the polysaccharide yield of purified polysaccharide crude products is 77.9% at least.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides, in particular to a method for extracting polysaccharides from Artemisia chinensis, belonging to the field of biomedicine. Background technique [0002] Polysaccharide (PS) is a macromolecule formed by the polymerization of aldose or ketose through glycosidic bonds, and widely exists in animals, plants and microorganisms. As an important living substance, polysaccharide has various biological activities such as anti-oxidation, anti-aging, anti-virus, anti-tumor, anti-fatigue, and hypoglycemia. Nowadays, many research results have shown that carbohydrates, as information molecules, play an important role in fertilization, development, development and differentiation, and maintenance of the nervous system. In addition, carbohydrates are also mediated in biological processes such as inflammation and autoimmune diseases, aging, abnormal proliferation and transformation of cancer cells, cell ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/006C08B37/0003
Inventor 刘春兰包瑛岳丽君黄雅婷申翔宇
Owner MINZU UNIVERSITY OF CHINA
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