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Liquid-state NAG (glucosaminidase) correction solution and preparation method thereof

A calibration liquid and liquid technology, applied in the field of NAG calibration liquid, can solve the problems of increasing the cost of biochemical diagnostic reagents, harsh storage conditions, and inconvenience for users, and achieve great social and economic benefits, good stability, and simple preparation Effect

Active Publication Date: 2017-05-31
GUANGZHOU KEFEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, fluorescence spectrophotometry or ultraviolet kinetic analysis is mostly used for the determination of NAG. The calibration solution used in the above determination method is a freeze-dried powder, which needs to be redissolved before each use, and the storage conditions are also relatively harsh, so it is very difficult for users. Caused a lot of inconvenience, also increased the use cost of this type of biochemical diagnostic reagent

Method used

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  • Liquid-state NAG (glucosaminidase) correction solution and preparation method thereof
  • Liquid-state NAG (glucosaminidase) correction solution and preparation method thereof
  • Liquid-state NAG (glucosaminidase) correction solution and preparation method thereof

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Embodiment 1

[0027] Configure 1L liquid NAG calibration solution of the present invention, the method is as follows:

[0028] Weigh each component according to the following formula:

[0029]

[0030] Dissolve the above components in 900mL deionized water, stir and dissolve fully, adjust the pH value to 7.4 to obtain the NAG calibration solution dilution; then add 45Uβ-N-acetylglucosaminidase to the NAG calibration solution dilution, and use Dilute to 1L with deionized water to obtain the NAG calibration solution; filter it through a 0.22μm filter membrane and store it at 2-8°C.

Embodiment 2

[0032] Configure 1L liquid NAG calibration solution of the present invention, the method is as follows:

[0033] Weigh each component according to the following formula:

[0034]

[0035] Dissolve the above components in 900mL deionized water, stir and dissolve fully, adjust the pH value to 6.3 to obtain the NAG calibration solution dilution, then add 45Uβ-N-acetylglucosaminidase to the NAG calibration solution dilution, and use Dilute to 1L with deionized water to obtain the NAG calibration solution; filter it through a 0.22μm filter membrane and store it at 2-8°C.

Embodiment 3

[0037] Configure 1L liquid NAG calibration solution of the present invention, the method is as follows:

[0038] Weigh each component according to the following formula:

[0039]

[0040] Dissolve the above components in 900mL deionized water, stir and dissolve fully, adjust the pH value to 7.6 to obtain the NAG calibration solution dilution, then add 45Uβ-N-acetylglucosaminidase to the NAG calibration solution dilution, and use Dilute to 1L with deionized water to obtain the NAG calibration solution; filter it through a 0.22μm filter membrane and store it at 2-8°C.

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Abstract

The invention disclsoes a liquid-state NAG (glucosaminidase) correction solution. The correction solution is prepared from the following components: 0.13-13g / L of sodium dihydrogen phosphate dihydrate, 1.26-12.6g / L of disodium hydrogen phosphate dodecahydrate, 0.1-20g / L of sodium chloride, 0.05-3g / L of potassium chloride, 0.5-10g / L of BSA (bull serum albumin), 0.1-10g / L of a surfactant, 0.1-3g / L of a preservative, 50-200g / L of a protein stabilizer and 45U / L of [beta]-N-acetylglucosaminidase; and pH value of the NGA correction solution is 6.0-8.0. With the application of the liquid-state NAG correction solution provided by the invention, the shortcoming of a conventional correction solution which needs to be preserved in a freeze-dried mode is overcome, and preservation conditions of the correction solution are improved, so that item detection in the medical field are facilitated.

Description

technical field [0001] The invention relates to the field of NAG calibration solution, in particular to a fully automatic liquid calibration solution for β-N-acetylglucosaminidase. Background technique [0002] β-N-acetylglucosaminidase (NAG) is a high-molecular-weight (140,000d) glycoprotein acid hydrolase, widely present in lysosomes of various tissues and organs, especially NAG content in renal proximal epithelial cells Very rich. However, due to its large molecular weight, NAG in serum generally cannot pass through glomerular filtration, so the content is relatively high in nephrons, especially in proximal convoluted tubular epithelial cells. [0003] When the renal tubules are damaged or slightly damaged in the early stage, the filtration and absorption of NAG by renal tubular cells are changed, causing the activity of NAG in the urine to increase significantly, and the changes are much earlier than anhydride urea nitrogen, urine muscle, urine Abnormal concentration o...

Claims

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Application Information

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IPC IPC(8): C12Q1/34
CPCC12Q1/34G01N2333/942
Inventor 刘丹丹何明深
Owner GUANGZHOU KEFEN BIOTECH CO LTD
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