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Pharmaceutical composition containing spirulina maxima extract as active ingredient for preventing and treating retinal diseases

A technology for retinal diseases, Spirulina maxima, applied in the field of pharmaceutical compositions for the prevention and treatment of retinal diseases containing extracts of Spirulina maxima as active ingredients, capable of solving blindness, neurons not working normally, neurasthenia And other issues

Inactive Publication Date: 2017-05-31
杰创有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So, neurons don't work properly
If this problem is ignored, it leads to permanent nervous breakdown, leading to blindness

Method used

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  • Pharmaceutical composition containing spirulina maxima extract as active ingredient for preventing and treating retinal diseases
  • Pharmaceutical composition containing spirulina maxima extract as active ingredient for preventing and treating retinal diseases
  • Pharmaceutical composition containing spirulina maxima extract as active ingredient for preventing and treating retinal diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Example 1: Preparation of Spirulina maxima extract

[0081] Spirulina maxima (Korea Marine Microalgae Cultivation Center registration number: KMMCC-1057) comes from the Korean Marine Microalgae Cultivation Center of the Ministry of Marine Biological Materials and Aquaculture, South Korea, and Pukyung University.

[0082] Specifically, the cultivated Spirulina maxima was centrifuged at 3000 rpm for 25 minutes in a multi-tube carrier refrigerated centrifuge (Vision Scientific CO. Ltd). The separated cells were briefly washed with 1.0% NaCl solution and then centrifuged again. The obtained cells were freeze-dried and used as a sample for phycocyanin extraction. The extraction was performed as follows: 10 mL of 0.1 M phosphate buffer (pH 7.0) was added to 40 mg of freeze-dried sample, and then vortexed for 15 minutes. The supernatant was obtained by centrifugation (3500 rpm, 5 minutes), which was used as an extract of Spirulina maxima.

Embodiment 2

[0083] Example 2: Preparation of allophycocyanin, R-phycoerythrin and C-phycocyanin

[0084] Allophycocyanin (A7472), R-phycoerythrin (P6161) and C-phycocyanin were purchased from Sigma-Aldrich, and they were supplied at 4mg / ml, 10mg / ml and 1mg / ml, respectively The concentration of preparation.

Embodiment 3

[0085] Example 3: Cell culture

[0086] The human adult ARPE cells (ARPE-19: catalog number CRL-2302) used in the experiments and analyses of the present invention were from the Vision Science Research Center of the Catholic University of Korea. Put the above ARPE cells in DMEM supplemented with 10% FBS, 100U / ml penicillin and 100mg / ml streptomycin, and in 5% CO 2 , Cultivate in an incubator at 37°C. Divide the cells to 5 cells or less 4 The density of cells / well was seeded in 6-well plates for further experiments.

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PUM

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Abstract

A Spirulina maxima extract of the present invention and Allophycocyanin (APC), R-phycoerythrin (R-PE), and C-phycocyanin (C-PC), which are components of the Spirulina maxima extract, show an effect of inhibiting cell death and A2E (pyridinium bis-retinoid), which is oxidized with a blue light, so that the present invention can be usefully applied as an active ingredient in a composition for preventing and treating retinal diseases.

Description

Technical field [0001] The present invention relates to a pharmaceutical composition containing spirulina maxima extract as an active ingredient for preventing and treating retinal diseases. Background technique [0002] People in modern society are living in the flood of visual culture. At least 80-90% of life information is obtained through eye experience. As a result, people's eyesight continues to decrease, indicating that the speed of vision decline caused by environmental reasons continues to increase. Modern people spend most of their time facing various digital screens such as computers and smart phones. The problem is that LEDs used in screens of digital devices such as smartphones emit blue light that threatens eye health. Blue light is light with blue color detected in 400-500 nm among visible light, such as light emitted from a digital device or a smart phone. When a digital device is turned on at night, even if the screen is truly white, its overall appearance is b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/02A61P27/02A61P27/00
CPCA61K2236/00A61K38/164A61P27/00A61P27/02A23L33/105A23V2002/00A61K35/748
Inventor 丁世荣金东俊梁承元崔相昊康徒衡许守镇
Owner 杰创有限公司
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